Research Article

Status resistensi larva nyamuk Aedes aegypti terhadap temefos di Desa Peguyangan Kaja, Kota Denpasar tahun 2020

Ida Bagus Putra Adyatma , Putu Ayu Asri Damayanti, I Kadek Swastika

Ida Bagus Putra Adyatma
Program Studi Pendidikan Dokter, Fakultas Kedokteran Universitas Udayana. Email: putraadyatma060@gmail.com

Putu Ayu Asri Damayanti
Departemen Parasitologi, Fakultas Kedokteran Universitas Udayana

I Kadek Swastika
Departemen Parasitologi, Fakultas Kedokteran Universitas Udayana
Online First: April 30, 2021 | Cite this Article
Adyatma, I., Damayanti, P., Swastika, I. 2021. Status resistensi larva nyamuk Aedes aegypti terhadap temefos di Desa Peguyangan Kaja, Kota Denpasar tahun 2020. Intisari Sains Medis 12(1): 294-297. DOI:10.15562/ism.v12i1.944


Introduction: Temephos 1% larvicide (Abate 1SG) has been used en mass and for a long time in an effort to control the Ae. aegypti mosquito population. Due to that usage, it might have induced a certain degree of resistance. The purpose of this study was to determine the resistance status, the temephos lethal concentration for the 50% (LC50) and 99% (LC99) of the A. aegypti larvae population from Peguyangan Kaja Village, Denpasar.

Methods: This research is an experimental study to assess resistance status and determine the 24-hour LC50 and LC99 values with control and 4 treatment groups, namely the temephos concentration group 0.012 mg/l; 0.025 mg/l; 0.125 mg/l; and 0.625 mg/l. Resistance testing is carried out by biological testing according to WHO standard.

Results: The results showed that the percentage of mortality of A. aegypti larvae on exposure to temephos with WHO diagnostic concentration (0.012 mg/l) was 54%. The results of the probit analysis showed that the LC50 24 hours ranged from 0.003-0.017 mg/l with an average of 0.011 mg/l, while the 24-hour LC99 ranged from 0.049-13.64 mg/l with an average of 0.112 mg/l.

Conclusion: This research shows that A. aegypti larvae in Peguyangan Kaja Village, Denpasar have shown resistance against temephos larvicide.

 

 

Pendahuluan: Larvasida temefos 1% (Abate 1SG) telah digunakan secara massal dan dalam jangka waktu yang lama dalam upaya pengendalian populasi nyamuk A. aegypti.  Hal ini dapat memicu terjadinya resistensi. Tujuan penelitian ini adalah untuk mengetahui status resistensi, nilai konsentrasi temefos yang efektif membunuh 50% (LC50) dan 99% (LC99) larva dari larvasida temefos terhadap populasi larva A. aegypti dari Desa Peguyangan Kaja, Kota Denpasar.

Metode: Penelitian ini merupakan studi eksperimental untuk menilai status resistensi dan mengetahui nilai LC50 dan LC99 24 jam dengan kontrol dan 4 kelompok perlakuan yaitu kelompok konsentrasi temefos 0.012 mg/l; 0.025 mg/l; 0.125 mg/l; dan 0.625 mg/l. Uji resistensi dilakukan dengan uji hayati sesuai standar WHO.

Hasil: Hasil penelitian menunjukkan persentase kematian larva A. aegypti terhadap paparan temefos dengan konsentrasi diagnostik WHO (0,012 mg/l) sebesar 54%. Hasil analisis probit menunjukkan bahwa nilai LC50 24 jam berkisar antara 0,003-0,017 mg/l dengan rata-rata 0,011 mg/l, sedangkan LC99 24 jam berkisar antara 0,049-13,64 mg/l dengan rata-rata 0,112 mg/l.

Simpulan: Penelitian ini menunjukkan bahwa larva A. aegypti di Desa Peguyangan Kaja, Kota Denpasar telah memiliki sifat resisten terhadap larvasida temefos.

References

Kementerian Kesehatan Republik Indonesia. 2020. Profil Kesehatan Indonesia Tahun 2019. Jakarta: Kemenkes RI. Tersedia di: https://pusdatin.kemkes.go.id/resources/download/pusdatin/profil-kesehatan-indonesia/Profil-Kesehatan-indonesia-2019.pdf

World Health Organization. Temephos in Drinking-water: Use for Vector Control in Drinking-water Sources and Containers. 2009. [sumber online]. diakses pada: 16 Januari 2021. Tersedia di: https://www.who.int/water_sanitation_health/dwq/chemicals/temephos.pdf

Braga IA, Lima JBP, Soares SS, Valle AD. Aedes aegypti Resistance to Temephos during 2001 in Several Municipalities in the States of Rio de Janeiro, Sergipe, and Alagoas, Brazil. Memórias do Instituto Oswaldo Cruz. 2004;99(2):199-203. Tersedia di: https://pubmed.ncbi.nlm.nih.gov/15250476/

Ponlawat A, Scott JG, Harrington LC. Insecticide susceptibility of Aedes aegypti and Aedes albopictus across Thailand. Journal of Medical Entomology. 2005;42(5):821-825. Tersedia di: https://academic.oup.com/jme/article/42/5/821/863586

Chen, Nazni WA, Lee HL, Sofian AM. Weekly variation on susceptibility status of Aedes mosquitoes against temephos in Selangor, Malaysia. Tropical Biomedicine. 2005;22(2):195-206. Tersedia di: https://pubmed.ncbi.nlm.nih.gov/16883288/

Muthusamy R, Shivakumar MS. Susceptibility status of Aedes aegypti (L.) (Diptera: Culicidae) to temephos from three districts of Tamil Nadu, India. Journal of vector borne diseases. 2015;52(2):159–65. Tersedia di: https://pubmed.ncbi.nlm.nih.gov/26119549/

Prasetyowati H, Hendri J, Wahono T. Status Resistensi Aedes aegypti (Linn.) terhadap Organofosfat di Tiga Kotamadya DKI Jakarta. Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara. 2016;12(1):23-30. Tersedia di: http://ejournal.litbang.kemkes.go.id/index.php/blb/article/view/4454

Mulyatno KC, Yamanaka A, Ngadino, Konishi E. Resistance of Aedes aegypti (L.) Larvae to temephos in Surabaya, Indonesia. Southeast Asian Journal of Tropical Medicine and Public Health. 2012;43(1):29-33. Tersedia di: https://pubmed.ncbi.nlm.nih.gov/23082551/

Istiana, Farida H, Isnaini. Resistance Status of Aedes aegypti larvae to temephos in West Banjarmasin. Jurnal BUSKI. 2012;4(2):53-58. Tersedia di: https://www.neliti.com/publications/21419/resistance-status-of-aedes-aegypti-larvae-to-temephos-in-west-banjarmasin

Naswin, Arimaswati, Alifariki LO, Bangu. Status Kerentanan Larva Aedes spp. Terhadap Insektisida Organofosfat di Kecamatan Poleang Timur. Indonesian Journal for Health Sciences. 2020;4(2):108-14. Tersedia di: http://journal.umpo.ac.id/index.php/IJHS/article/view/2383

World Health Organization. Guidelines for laboratory and Field Testing of Mosquito larvicides. 2005. [sumber online]. diakses pada: 16 Januari 2021. Tersedia di: https://apps.who.int/iris/bitstream/10665/69101/1/WHO_CDS_

WHOPES_GCDPP_2005.13.pdf

World Health Organization. Monitoring and managing insecticide resistance in Aedes mosquito populations: interim guidance for entomologists. Geneva; 2016. Tersedia di: https://apps.who.int/iris/handle/10665/204588

Taslisia T, Rusjdi SR, Hasmiwati. 2018. Survei Entomologi, Maya Indeks, dan Status Kerentanan Larva Nyamuk Ae. aegypti terhadap temephos. Jurnal Kesehatan Andalas. 2018;7(1):33-41. Tersedia di: http://jurnal.fk.unand.ac.id/index.php/jka/article/view/777

Fenisenda A, Rahman AO. Uji Resistensi Larva Nyamuk Aedes aegypti terhadap Abate (temephos) 1% di Kelurahan Mayang Mangurai Kota Jambi pada Tahun 2016. Jambi Medical Journal. 2016;4(2):101-105. Tersedia di: https://online-journal.unja.ac.id/index.php/kedokteran/article/view/3576

Purwantisari S, Martini M, Santoso L, Handayani N. Status Resistensi Larva Aedes aegypti terhadap temephos di Wilayah Perimeter dan Buffer Pelabuhan Tanjung Emas Kota Semarang. Jurnal Kesehatan Masyarakat Universitas Diponegoro. 2016;4(1):159-166. Tersedia di: https://media.neliti.com/media/publications/18512-ID-status-resistensi-larva-aedes-aegypti-terhadap-temephos-di-wilayah-perimeter-dan.pdf

World Health Organization. Global Plan for Insecticide Resistance Management in Malaria Vectors. 2012. [sumber online]. diakses pada: 16 Januari 2021. Tersedia di: http://www.who.int/malaria/publications/atoz/gpirm

Insecticide Resistance Action Committee (IRAC). Prevention and Management of Insecticide Resistance in Vectors of Public Health Importance. 2011. [sumber online]. diakses pada: 16 Januari 2021. Tersedia di: http://www.irac-online.org/content/uploads/VM-layout-v2.6_LR.pdf

Perwitasari D, Musadad DA, Manalu HS, Munif A. Pengaruh Beberapa Dosis Bacillus Thuringiensis Var Israelensis Serotype H14 Terhadap Larva Ae. aegypti Di Kalimantan Barat. Jurnal Ekologi Kesehatan. 2015;14(3):229-37. Tersedia di: http://ejournal.litbang.kemkes.go.id/index.php/jek/article/view/4693


No Supplementary Material available for this article.
Article Views      : 21
PDF Downloads : 8