Detection of biofilm formation in clinical isolates of Streptococcus pneumoniae in Sanglah General Hospital, Bali, Indonesia
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- DOI: https://doi.org/10.15562/ism.v12i1.908 |
- Published: 2021-04-30
Open Access & Peer Reviewed
Multidisciplinary Journal of Science and
Medical Research
Multidisciplinary Journal of Science and
Medical Research
Abstract
Background: Streptococcus pneumoniae causes broad-spectrum infections from mild to severe with high morbidity and mortality rates in almost all of the world, namely pneumonia and meningitis. This bacterium has virulence factors that help their survival, one of which is biofilms. Biofilms help Streptococcus pneumoniae become resistant to antibiotics; thus, treating infections caused by these bacteria is difficult to treat. This study aims to determine the biofilm production ability of Streptococcus pneumoniae isolated from the Clinical Microbiology Laboratory of Sanglah General Hospital, Denpasar, Bali, Indonesia using the tissue culture plate method.
Methods: The research design used was a descriptive observational study with cross sectional type. The clinical isolate of Streptococcus pneumoniae was isolated from the Clinical Microbiology Laboratory of Sanglah General Hospital. Biofilm formation was measured by the tissue culture plate method and carried out at the Microbiology Laboratory of the Faculty of Medicine, Udayana University. Data were analyzed using SPSS version 20 for Windows.
Results: Most of the specimens were collected from blood (59.37%), followed by sputum (31.25%), and others (9.38%). It was found that 1 of 32 (3.10%) clinical isolates could form a biofilm with a strong formation category (the optical density value> 0.38). In contrast, the rest did not form biofilms with an optical density value of ?0.095.
Conclusions: Not all clinical isolates of Streptococcus pneumoniae isolated from the Clinical Microbiology Laboratory of Sanglah General Hospital Denpasar were able to form biofilms, suggesting that other virulence factors also play a role in pneumococcal infection. However, a molecular approach is necessary for the detection of genes encoding biofilm-producing isolates in future studies.
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2. Bénet T, Picot VS, Awasthi S, Pandey N, Bavdekar A, Kawade A, et al. Severity of Pneumonia in Under 5-Year-Old Children from Developing Countries: A Multicenter, Prospective, Observational Study. Am J Trop Med Hyg. 2017 Jul;97(1):68-76.
3. Dinas Kesehatan Provinsi Bali. Profil Kesehatan Provinsi Bali 2017 [Internet]. 2017. [Available from: https://diskes.baliprov.go.id/wp-content/uploads/2019/06/Bali_Profil_2017_ds.pdf]
4. Brouwer MC, Tunkel AR, van de Beek D. Epidemiology, diagnosis, and antimicrobial treatment of acute bacterial meningitis. Clin Microbiol Rev. 2010;23(3):467-492.
5. van de Beek D, Brouwer M, Hasbun R, Koedel U, Whitney CG, Wijdicks E. Community-acquired bacterial meningitis. Nat Rev Dis Primers. 2016;2:16074.
6. Saputro A, Farida H, Firmanti S. Perbedaan Pola Kepekaan Terhadap Antibiotik pada Streptococcus pneumoniae yang Mengkolonisasi Nasofaring Balita. J Kedokt Diponegoro. 2013;2(1):137734.
7. Brooks LRK, Mias GI. Streptococcus pneumoniae's Virulence and Host Immunity: Aging, Diagnostics, and Prevention. Front Immunol. 2018;9:1366.
8. Chao Y, Marks LR, Pettigrew MM, Hakansson AP. Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease. Front Cell Infect Microbiol. 2015;4:194.
9. Shakibaie MR. Bacterial Biofilm and its Clinical Implications. Annals of Microbiology and Research. 2018;2(1):45–50.
10. Wu H, Moser C, Wang HZ, Høiby N, Song ZJ. Strategies for combating bacterial biofilm infections. Int J Oral Sci. 2015;7(1):1-7.
11. Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1985;22(6):996-1006.
12. Wróblewska J, Bia?ucha A, Kubik EA, Gospodarek-Komkowska E. Biofilm formation of Streptococcus pneumoniae from bronchial alveolar lavage and from nasal swab. Med Res J. 2017;1(3):100–4.
13. Yadav MK, Chae SW, Song JJ. In VitroStreptococcus pneumoniae Biofilm Formation and In Vivo Middle Ear Mucosal Biofilm in a Rat Model of Acute Otitis Induced by S. pneumoniae. Clin Exp Otorhinolaryngol. 2012;5(3):139-144.
14. García-Castillo M, Morosini MI, Valverde A, Almaraz F, Baquero F, Cantón R, et al. Differences in biofilm development and antibiotic susceptibility among Streptococcus pneumoniae isolates from cystic fibrosis samples and blood cultures. J Antimicrob Chemother. 2007;59(2):301-4.
15. Furtuna DK, Debora K, Wasito EB. Antimicrobial susceptibility and the pattern of a biofilm-forming pair of organisms from patients treated in intensive care units in Dr. Soetomo General Hospital, Indonesia. Bali Medical Journal. 2019;8(1):51-58.
16. Camilli R, Pantosti A, Baldassarri L. Contribution of serotype and genetic background to biofilm formation by Streptococcus pneumoniae. Eur J Clin Microbiol Infect Dis. 2011;30(1):97-102.
How to Cite
Vijayananda, I. M. S., Hendrayana, M. A., Sukrama, I. D. M., & Fatmawati, N. N. D. (2021). Detection of biofilm formation in clinical isolates of Streptococcus pneumoniae in Sanglah General Hospital, Bali, Indonesia. Intisari Sains Medis, 12(1), 356–359. https://doi.org/10.15562/ism.v12i1.908
