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Prevalensi gen penyandi resisten Colistin mcr-1 pada isolat Klebsiella pneumoniae dan Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) di RSUP Sanglah, Bali tahun 2018


Background: Antimicrobial resistance (AMR) is a serious threat to health, both human and animal health. Antibiotic resistance in the Enterobacteriaceae family, especially the bacteria Klebsiella pneumoniae and Escherichia coli, is a special focus because it is caused by pathogenic Gram-negative bacteria found in humans. This study aims to study the prevalence of Colistin mcr-1 resistant coding gene in Klebsiella pneumoniae and Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) isolates in Sanglah Hospital, Bali, in 2018.

Methods: This study was conducted in 2 stages, namely examination by PCR and telephone by sequencing. This study's object was the bacteria Klebsiella pneumoniae and Escherichia coli ESBL in clinical isolates of the Clinical Microbiology Laboratory of Sanglah Hospital Denpasar-Bali. Data were analyzed using SPSS version 20 for Windows.

Results: The results of this study indicate that the results of clinical examination of 300 isolates, most of the K. pneumoniae and E. coli specimens were found in urine (32.00%), followed by pus (24.00%), sputum (20.00%), blood (12.00%), and others (11.00%). Most of the K. pneumoniae infections were found in sputum (70.49%), pus (52.05%), and blood (70.27%) specimens. Of the 300 isolates obtained from the results, no isolates had the gene encoding colistin mcr-1 resistance in clinical isolates tested molecularly.

Conclusion: From the results of this study, it can be concluded that the colistin mcr-1 resistance coding gene was not found in the tested isolates.



Latar Belakang: Antimicrobial resistance (AMR) merupakan ancaman serius di dunia kesehatan baik kesehatan manusia maupun hewan. Resistensi antibiotika pada family Enterobacteriaceae khususnya bakteri Klebsiella pneumoniae dan Escherichia coli menjadi fokus khusus karena keduanya merupakan bakteri Gram negatif patogen yang sering ditemukan pada manusia. Penelitian ini bertujuan untuk mengevaluasi prevalensi gen penyandi resisten Colistin mcr-1 pada isolat Klebsiella pneumoniae dan Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) di RSUP Sanglah, Bali tahun 2018

Metode: Penelitian ini dilakukan dalam 2 tahap yaitu identifikasi dengan PCR dan identifikasi dengan sekuensing. Objek dari penelitian ini ada bakteri Klebsiella pneumoniae dan Escherichia coli ESBL pada isolat klinik Laboratorium Mikrobiologi Klinik RSUP Sanglah Denpasar-Bali. Data dianalisis dengan SPSS versi 20 untuk Windows.

Hasil: Hasil penelitian ini menunjukkan bahwa hasil pemeriksaan terhadap 300 isolat klinis sebagian besar specimen K. pneumoniae dan E. coli ditemukan pada urin (32,00%), diikuti dengan pus (24,00%), sputum (20,00%), darah (12,00%), dan lain-lain (11,00%). Sebagian besar infeksi K. pneumoniae ditemukan pada spesimen sputum (70,49%), pus (52,05%), dan darah (70,27%). Dari 300 isolat yang diperiksa didapatkan hasil bahwa tidak ada isolat yang memiliki gen penyandi resistensi colistin mcr-1 pada isolat klinik yang diujikan secara molekuler.

Kesimpulan: Dari hasil penelitian ini dapat disimpulkan bahwa gen penyandi resisten colistin mcr-1 tidak ditemukan pada isolat yang diuji


  1. Trecarichi EM, Pagano L, Candoni A, Pastore D, Cattaneo C, Fanci R, et al. Current epidemiology and antimicrobial resistance data for bacterial bloodstream infections in patients with hematologic malignancies: an Italian multicentre prospective survey. Clin Microbiol Infect. 2015;21(4):337-43.
  2. Stewardson AJ, Renzi G, Maury N, Vaudaux C, Brossier C, Fritsch E, et al. Extended-spectrum ?-lactamase-producing Enterobacteriaceae in hospital food: a risk assessment. Infect Control Hosp Epidemiol. 2014;35(4):375-83.
  3. Yao X, Doi Y, Zeng L, Lv L, Liu JH. Carbapenem-resistant and colistin-resistant Escherichia coli co-producing NDM-9 and MCR-1. Lancet Infect Dis. 2016;16(3):288-9.
  4. Eiamphungporn W, Yainoy S, Jumderm C, Tan-Arsuwongkul R, Tiengrim S, Thamlikitkul V. Prevalence of the colistin resistance gene mcr-1 in colistin-resistant Escherichia coli and Klebsiella pneumoniae isolated from humans in Thailand. J Glob Antimicrob Resist. 2018;15:32-35.
  5. Lalaoui R, Djukovic A, Bakour S, Sanz J, Gonzalez-Barbera EM, Salavert M, et al. Detection of plasmid-mediated colistin resistance, mcr-1 gene, in Escherichia coli isolated from high-risk patients with acute leukemia in Spain. J Infect Chemother. 2019;25(8):605-609.
  6. Walsh PS, Metzger DA, Higushi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques. 2013;54(3):134-139.
  7. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-8.
  8. Cao L, Li X, Xu Y, Shen J. Prevalence and molecular characteristics of mcr-1 colistin resistance in Escherichia coli: isolates of clinical infection from a Chinese University Hospital. Infect Drug Resist. 2018;11:1597-1603.
  9. Wise MG, Estabrook MA, Sahm DF, Stone GG, Kazmierczak KM. Prevalence of mcr-type genes among colistin-resistant Enterobacteriaceae collected in 2014-2016 as part of the INFORM global surveillance program. PLoS One. 2018;13(4):e0195281.
  10. Schwarz S, Johnson AP. Transferable resistance to colistin: a new but old threat. J Antimicrob Chemother. 2016;71(8):2066-2070.
  11. Robin F, Beyrouthy R, Colot J, Saint-Sardos P, Berger-Carbonne A, Dalmasso G, et al. MCR-1 in ESBL-producing Escherichia coli responsible for human infections in New Caledonia. J Antimicrob Chemother. 2017;72(3):946-947.
  12. Bilal H, Hameed F, Khan MA, Khan S, Yang X, Rehman T. Detection of mcr-1 Gene in Extended-Spectrum ?-Lactamase-Producing Klebsiella pneumoniae From Human Urine Samples in Pakistan. Jundishapur Journal of Microbiology. 2020;13(4):e96646
  13. Snesrud E, He S, Chandler M, Dekker JP, Hickman AB, McGann P, et al. A Model for Transposition of the Colistin Resistance Gene mcr-1 by ISApl1. Antimicrob Agents Chemother. 2016;60(11):6973-6976.

How to Cite

Violentina, G. A. D., Budayanti, N. N. S., & Iswari, I. S. (2020). Prevalensi gen penyandi resisten Colistin mcr-1 pada isolat Klebsiella pneumoniae dan Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) di RSUP Sanglah, Bali tahun 2018. Intisari Sains Medis, 11(3), 1537–1540.




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Gusti Ayu Dianti Violentina
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Ni Nyoman Sri Budayanti
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