Research Article

Ekstrak buah makasar (Brucea Javanica (L) Mer.) meningkatkan kadar enzim Superoksida Dismustase (SOD) mencit (Mus musculus) Swiss Webster jantan yang diinduksi paraquat

Maria Eka Patri Yulianti

Maria Eka Patri Yulianti
Departemen Ilmu Biologi, Fakultas Kedokteran dan Ilmu Kesehatan Universitas Bengkulu, Bengkulu, Indonesia. Email: mariaekapy18@gmail.com
Online First: October 13, 2020 | Cite this Article
Yulianti, M. 2020. Ekstrak buah makasar (Brucea Javanica (L) Mer.) meningkatkan kadar enzim Superoksida Dismustase (SOD) mencit (Mus musculus) Swiss Webster jantan yang diinduksi paraquat. Intisari Sains Medis 11(3): 675-678. DOI:10.15562/ism.v11i3.743


Background: Aging is a biological process where one of the contributing factors is oxidative stress due to free radicals in the body. Paraquat is an example of free radicals that are often used by oil palm plantation farmers in Bengkulu, which can trigger the aging process by reducing the enzyme Superoxide Dismutase (SOD). The provision of fruit extract of Makassar (Brucea javanica (L) Mer) contains bioactive compounds that can function as antioxidants by increasing SOD levels in the body. This study aims to prove that the fruit extract can increase the levels of SOD of male Swiss Webster mice induced by paraquat.

Methods: In vivo experimental research with randomized pretest-posttest control group design was conducted on 14 Swiss Webster mice (Mus musculus) as research subjects. The research subjects were then divided into 2 groups, namely the control and the treatment group (20 mg/kgBW/day (P1) for 14 days). Data were analyzed using SPSS version 17 for Windows.

Results: The results showed that there was no significant increase in the mean of SOD levels either before (16.97±1.45 U/ml) or after (17.07±1.89 U/ml) treatment in the P0 group (p> 0.05). However, in the P1 group that was given paraquat induction coupled with Makassar fruit extract at a dose of 20 mg/kgBW/day for 14 days, it showed that there was a significant increase in the mean SOD levels from 17.18±1.69 U/ml to 67.56±3.65 U/ml (p <0.05).

Conclusion: Provision of Makasar fruit extract at a dose of 20 mg/kgBW/day for 14 days was found to significantly increase the mean SOD levels in Swiss Webster male mice induced by paraquat.

 

Latar Belakang: Penuaan adalah suatu proses biologi dimana salah satu faktor penyebabnya adalah stres oksidatif akibat radikal bebas yang terjadi di dalam tubuh. Paraquat merupakan salah satu contoh radikal bebas yang sering digunakan oleh petani perkebunan sawit di Bengkulu dimana dapat memicu proses penuaan melalui penurunan kadar enzim Superoksida Dismutase (SOD). Pemberian ekstrak buah makasar (Brucea javanica (L) Mer) mengandung senyawa bioaktif yang dapat berfungsi sebagai antioksidan dengan meningkatkan kadar SOD dalam tubuh. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak buah makasar dapat meningkatkan kadar SOD mencit jantan swiss Webster yang diinduksi paraquat.

Metode: Penelitian eksperimental secara in vivo dengan rancangan randomized pretest-posttest control group design dilakukan terhadap 14 ekor mencit (Mus musculus) swiss Webster sebagai subjek penelitian. Subjek penelitian kemudian dibagi menjadi 2 kelompok, yaitu kelompok kontrol dan kelompok perlakuan (20 mg/kgBB/hari (P1) selama 14 hari). Data dianalisis dengan SPSS versi 17 untuk Windows.

Hasil: Hasil penelitian menunjukan bahwa tidak terdapat peningkatan bermakna rerata kadar SOD baik sebelum (16,97±1,45 U/ml) atau setelah (17,07±1,89 U/ml) perlakuan pada kelompok P0 (p>0,05). Akan tetapi, pada kelompok P1 yang diberikan induksi paraquat ditambah dengan ekstrak buah makasar dengan dosis 20 mg/kgBB/hari selama 14 hari menujukkan terdapat peningkatan secara bermakna rerata kadar SOD dari 17,18±1,69 U/ml menjadi 67,56±3,65 U/ml (p<0,05).

Kesimpulan: Pemberian ekstrak buah makasar dosis 20 mg/kgBB/hari selama 14 hari diketahui dapat meningkatkan rerata kadar SOD secara bermakna pada mencit jantan swiss Webster yang diinduksi paraquat.

References

Harman D. The aging process. Proc Natl Acad Sci U S A. 1981;78(11):7124-7128.

Niccoli T, Partridge L. Ageing as a risk factor for disease. Curr Biol. 2012;22(17):R741-R752.

Wickens AP. Ageing and the free radical theory. Respir Physiol. 2001;128(3):379-391.

Barja G. Free radicals and aging. Trends Neurosci. 2004;27(10):595-600.

Sekiyama M, Tanaka M, Gunawan B, Abdoellah O, Watanabe C. Pesticide usage and its association with health symptoms among farmers in rural villages in West Java, Indonesia. Environ Sci. 2007;14 Suppl:23-33.

Saravu K, Sekhar S, Pai A, Barkur AS, Rajesh V, Earla JR. Paraquat - A deadly poison: Report of a case and review. Indian J Crit Care Med. 2013;17(3):182-184.

Sukumar CA, Shanbhag V, Shastry AB. Paraquat: The Poison Potion. Indian J Crit Care Med. 2019;23(Suppl 4):S263-S266.

Muliarta M, Tirtayasa K, Prabawa PY, Wiryadana KA. Tamarillo Consumption Associated with Increased Acetylcholinesterase Activity and Improved Oxidative Stress Markers in Farmers Exposed to Daily Pesticide-related Activities in Baturiti, Bali, Indonesia. Open Access Macedonian Journal of Medical Sciences. 2020;8(E):244-250.

Iskandar, Utama SP, Barchia MF. Analisis Keberlanjutan Pengelolaan Perkebunan Kelapa Sawit Pola Inti-Plasma Di PT. Bio Nusantara Teknologi Kabupaten Bengkulu Tengah. Naturalis. 2018;7(1):1-8.

Wibawa W, Sugandi D. Herbisida Efektif, Efisien Dan Ramah Lingkungan Untuk Pengendalian Gulma Pada Perkebunan Kelapa Sawit Rakyat Di Provinsi Bengkulu. Balai Pengkajian Teknologi Pertanian (BPTP) Bengkulu. 2012.

Ghosh S, Singh A, Dewan H, Walia G, Bansal A. Herbicide poisoning: A diagnostic challenge. Indian J Crit Care Med. 2012;16(1):52-54.

Robertus Y, Anastasyah L, Tanoyo Y. Efek Siklofosfamid dan Steroid untuk penanganan Keracunan Parakuat. Journal Indonesian Medical Association. 2012;62(1):66-70.

Bismuth C, Garnier R, Baud FJ, Muszynski J, Keyes C. Paraquat poisoning. An overview of the current status. Drug Saf. 1990;5(4):243-251.

Blanco-Ayala T, Andérica-Romero AC, Pedraza-Chaverri J. New insights into antioxidant strategies against paraquat toxicity. Free Radic Res. 2014;48(6):623-640.

Ablat A, Mohamad J, Awang K, Shilpi JA, Arya A. Evaluation of antidiabetic and antioxidant properties of Brucea javanica seed. ScientificWorldJournal. 2014;2014:786130.

Ablat A, Halabi MF, Mohamad J, Hafiz M, Hasnan H, Hazni H, et al. Antidiabetic effects of Brucea javanica seeds in type 2 diabetic rats. BMC Complement Altern Med. 2017;17(1):94.

Wiemer M, Osiewacz HD. Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina. Microb Cell. 2014;1(7):225-240.

Zhao L, Li C, Zhang Y, Wen Q, Ren D. Phytochemical and biological activities of an anticancer plant medicine: Brucea javanica. Anticancer Agents Med Chem. 2014;14(3):440-458.

Bromilow RH. Paraquat and sustainable agriculture. Pest Manag Sci. 2004;60(4):340-349.

Suntres ZE. Role of antioxidants in paraquat toxicity. Toxicology. 2002;180(1):65-77.

Black AT, Gray JP, Shakarjian MP, Laskin DL, Heck DE, Laskin JD. Increased oxidative stress and antioxidant expression in mouse keratinocytes following exposure to paraquat. Toxicol Appl Pharmacol. 2008;231(3):384-392.

Wasserman B, Block ER. Prevention of acute paraquat toxicity in rats by superoxide dismutase. Aviat Space Environ Med. 1978;49(6):805-809.

Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;97:55-74.

Kim JA, Lau EK, Pan L, De Blanco EJ. NF-kappaB inhibitors from Brucea javanica exhibiting intracellular effects on reactive oxygen species. Anticancer Res. 2010;30(9):3295-3300.


No Supplementary Material available for this article.
Article Views      : 121
PDF Downloads : 34