Research Article

The effect of Euphorbia milii tea and its combination with Propolis on number of glomeruli in M.tb-infected mice: a histopathology study

Ruthirar Kalaichelvam , Ni Made Linawati, I Gusti Nyoman Sri Wiryawan, Gede Wirata, I Gusti Ayu Dewi Ratnayanti, I Wayan Sugiritama, Ida Ayu Ika Wahyuniari, I Gusti Kamasan Nyoman Arijana

Ruthirar Kalaichelvam
Bachelor of Medical Study Program, Medical Faculty, Udayana University. Email: ruthirarneo@gmail.com

Ni Made Linawati
Department of Histology, Medical Faculty, Udayana University

I Gusti Nyoman Sri Wiryawan
Department of Histology, Medical Faculty, Udayana University

Gede Wirata
Department of Anatomy, Medical Faculty, Udayana University

I Gusti Ayu Dewi Ratnayanti
Department of Histology, Medical Faculty, Udayana University

I Wayan Sugiritama
Department of Histology, Medical Faculty, Udayana University

Ida Ayu Ika Wahyuniari
Department of Histology, Medical Faculty, Udayana University

I Gusti Kamasan Nyoman Arijana
Department of Histology, Medical Faculty, Udayana University
Online First: December 01, 2019 | Cite this Article
Kalaichelvam, R., Linawati, N., Wiryawan, I., Wirata, G., Ratnayanti, I., Sugiritama, I., Wahyuniari, I., Arijana, I. 2019. The effect of Euphorbia milii tea and its combination with Propolis on number of glomeruli in M.tb-infected mice: a histopathology study. Intisari Sains Medis 10(3). DOI:10.15562/ism.v10i3.496


Background: The role of both Euphorbia milii and Propolis is to increase the effectiveness in pathological condition of the body part damaged, include renal function in maintaining the homeostasis. That combination into mice which are infected by Mycobacterium tuberculosis will change the total number of glomerulus in the kidney.

Aim: The study aims to determine the effect of combination tea from Euphorbia milii and Propolis on the number of the glomerulus in mice infected by M. tb.

Method: This study confirmed randomized post-test only control group design consisting of 24 mice divided into six groups (G1-G6). E. milli flowers are obtained from flower plantations, in Ketewel, Gianyar, Bali Province took in fresh and dark red sampling. M. tuberculosis used H37Rv strain (ATCC27294) grown on Middlebrook 7H9 liquid medium for two weeks.

Result: It was found that Group 3 and Group 5 from the treatment group has the most significant average number of glomerulus which was 13, followed by Group 2 and Group 6 from the control group has the largest average number of glomerulus which was 12. The differences were not significant between groups with P = 0.463 (P <0.05).

Conclusion: The Euphorbia milii tea and its combination with Propolis does not affect histological change on the total number of glomerulus in mice infected with M. tb.

References

Linawati, N. M., Sukrama, D. M., & Mertaniasih, M.(2014). The influence of Euphorbia milii Flower Extract in the activity of Th17 through IL-17 Secretion In Mycobacterium tuberculosis Infected Mice, International Journal of Science and Research, 626-627.

Tao, Y., Wang, D., Hu. Y., Yu, Y., Wang, D. 2014. The immunological Enhancement Activity of Propolis Flavonoids Liposome in Vitro and in Vivo. Hindawi Publishing

Lutz Slomianka. 2009. School of Anatomy and Human Biology - The University of Western Australia.

Saunders, B. M., & Cheers, C (1996). Intranasal Infection of Beige mice with Mycobacterium avium Complex: Role of neutrophils and natural killer cells. Infection and Immunity

Shuai, H., Z. Cui-Ping., W. Kai., Q.L. George and H. Fu Liang. 2014. Recent Advances in the Chemical Composition of Propolis. Molecules. 19:19610- 19632.

Cormack, D.W. Essential Histology Second Edition. Lippincott William & Wilkins. Pennsylvania. Philadelphia; 2001: 52.

Wirata, G., Santoso, P., Dewantari, P. 2018. Microstructural aspect of pineal body: pinealocytes population. Intisari Sains Medis 9(1). DOI:10.15562/ism.v9i1.151

Widyastuti, N.N.A., Bagiada, I.M., Andrika, P. 2019. Karakteristik penderita tuberkulosis paru relapse yang berobat di poli paru RSUP Sanglah Denpasar Bali periode Mei 2017 hingga September 2018. Intisari Sains Medis 10(2): 328-333. DOI: 10.15562/ism.v10i2.386

Artini, I., Artana, I., Aman, I., Mahendra, A. 2017. CYP2E1 genotype and transaminase level of tuberculosis patients receiving fixed dose combination of antituberculosis. Bali Medical Journal 6(3): S70-S74. DOI:10.15562/bmj.v6i3.731

Vijay DW. Propolis: A Wonder Bees Product and Its Pharmacological Potentials. Advances in Pharmacological Sciences, 2013; 2013: 1–11

Monika Bhadauria, “Propolis Prevents Hepatorenal Injury Induced by Chronic Exposure to Carbon Tetrachloride,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 235358, 12 pages, 2012. https://doi.org/10.1155/2012/235358.


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