Research Article

Pengaruh ekstrak krim Morinda citrifolia terhadap jumlah fibroblas pada penyembuhan luka tikus Wistar

Vania Lannisa Haestidyatami , I Wayan Sugiritama, Ni Made Linawati

Vania Lannisa Haestidyatami
Program Studi Pendidikan Dokter, Fakultas Kedokteran Universitas Udayana, Denpasar Bali. Email:

I Wayan Sugiritama
Departemen Histologi, Fakultas Kedokteran Universitas Udayana, Denpasar, Bali

Ni Made Linawati
Departemen Histologi, Fakultas Kedokteran Universitas Udayana, Denpasar, Bali
Online First: December 01, 2019 | Cite this Article
Haestidyatami, V., Sugiritama, I., Linawati, N. 2019. Pengaruh ekstrak krim Morinda citrifolia terhadap jumlah fibroblas pada penyembuhan luka tikus Wistar. Intisari Sains Medis 10(3). DOI:10.15562/ism.v10i3.487

Introduction. Wound healing is a complex biological process involving four stages, i.e., hemostasis, inflammation, proliferation, and remodeling. In the proliferation phase, there is an increase in the number of fibroblas cells. Morinda citrifolia is a fruit that contains the antioxidant compound, Quercetin. These antioxidant properties are thought to be able to accelerate the process of wound healing through the process of modulating the oxidative process and improving skin microstructure. The relationship of Quercetin to fibroblas cells which plays an essential role in the proliferation phase is not yet fully known. This study was conducted to determine the effect of Morinda citrifolia extracts on the number of fibroblas cells during the process of wound healing in Wistar rats.

Method. Experimental, post-test with control group study was conducted using 24 rats divided into four groups. In group I, rats were treated with incisions and given a Placebo cream, groups II, III and IV were given incisions and cream containing ethanol extract of Morinda citrifolia 5%, 10%, and 20% respectively. The number of fibroblass was calculated using the digital analysis method using Image Raster 3 Software. The number of fibroblas cells was calculated as the percentage of fibroblas cells seen in the field of view. Data analysis conducted with the One-Way ANOVA and Post-Hoc LSD test.

Results. The control group had an average number of fibroblas cells 23.3% ± 1.9%. The mean fibroblas cells in the intervention group with extracts of 5%, 10%, 20% were 27.6% ± 6.7%, 38.3% ± 8.8% and 22.3% ± 2.7%, respectively. The ANOVA test results showed a significant difference in the number of fibroblass between treatment groups (p<0.001). Post-hoc LSD test shown that only the treatment group with 10% extract showed a difference in the average number of fibroblas cells compared to the other groups.

Conclusion. The highest number of fibroblas cells was found in the treatment group with 10% extract.


Kalangi S. Histofisiologi Kulit. J Biomedik. 2013;5(3):S12-20.

Beanes SR, Dang C, Soo C, Ting K. Skin repair and scar formation: the central role of TGF-β. Expert Rev Mol Med. 2003;5(8):1–22. Diakses dari:

Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008;453(7193):314–21. Diakses dari:

Bielefeld KA, Amini-Nik S, Alman BA. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol Life Sci. 2013;70(12):2059–81. Diakses dari:

Sumbayak EM. Fibroblas: Struktur dan Peranannya dalam Penyembuhan Luka. J Kedokt Meditek. 2015;21(57):1–6.

Yuslianti ER, Sabirin IPR, B. Sutjiat A. Effectiveness of Noni (Morinda citrifolia L.) Leaves Extract Gel as Standardized Traditional Medicine to Accelerate Oral Mucosa Wound Healing on Wistar Rats. Res J Med Plant. 2014;8(4):156–66. Diakses dari:

Dueñas M, Surco-Laos F, González-Manzano S, González-Paramás AM, Santos-Buelga C. Antioxidant properties of major metabolites of quercetin. Eur Food Res Technol. 2011;232(1):103–11. Diakses dari:

Sarandy MM, Novaes RD, Xavier AA, Vital CE, Leite JP V., Melo FCSA, et al. Hydroethanolic Extract of Strychnos pseudoquina Accelerates Skin Wound Healing by Modulating the Oxidative Status and Microstructural Reorganization of Scar Tissue in Experimental Type I Diabetes. Biomed Res Int. 2017;2017:1–11. Diakses dari:

Sutrisno T, Huda N, Nurlely N, Cahaya N, Srikartika VM. Efektivitas Gel Kuersetin pada Penyembuhan Luka Bakar Derajat IIA. Media Pharm Indones . 2017;1(1):1. Diakses dari:

Sukrama DM, Wihandani DM, Manuaba AP, Manuaba AP. Topical Binahong (Anredera cordifolia) Leaf Extract Increases Interleukin-6 and VEGF (Vascular Endothelial Growth Factor) during Burn Wound Healing in Wistar Rats Infected with Pseudomonas aeruginosa. Biol Med. 2017;09(01):1–6. Diakses dari:

Putri RR, Hakim RF, Rezeki S. Pengaruh Ekstrak Daun Tapak Dara (Catharanthus Roseus) Terhadap Jumlah Fibroblas Pada Proses Penyembuhan Luka Di Mukosa Oral. J Caninus Dent. 2017;2(1):20–30. Diakses dari:

Birt DF, Jeffery E. Flavonoids. Adv Nutr. 2013;4(5):576–7. Diakses dari:

Lagunes I, Trigos Á. Photo-oxidation of ergosterol: Indirect detection of antioxidants photosensitizers or quenchers of singlet oxygen. J Photochem Photobiol B Biol. 2015;145:30–4. Diakses dari:

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