Research Article

Uji efektivitas ekstrak etanol kulit buah naga merah (Hylocereus polyrhizus) terhadap Staphylococcus aureus ATCC 25923 secara in vitro

Anak Agung Bintang Astridwiyanti , Agung Nova Mahendra, Ni Wayan Sucindra Dewi

Anak Agung Bintang Astridwiyanti
Program Studi Pendidikan Dokter, Fakultas Kedokteran, Universitas Udayana. Email: bintangastridwiyanti@gmail.com

Agung Nova Mahendra
Bagian Farmakologi, Fakultas Kedokteran Universitas Udayana

Ni Wayan Sucindra Dewi
Bagian Farmakologi, Fakultas Kedokteran Universitas Udayana
Online First: September 02, 2019 | Cite this Article
Astridwiyanti, A., Mahendra, A., Dewi, N. 2019. Uji efektivitas ekstrak etanol kulit buah naga merah (Hylocereus polyrhizus) terhadap Staphylococcus aureus ATCC 25923 secara in vitro. Intisari Sains Medis 10(3). DOI:10.15562/ism.v10i3.425


Introduction: Infectious diseases are still one of the main causes of high morbidity and mortality in tropical Indonesia. One of the causes of this disease is the staphylococcus aureus bacteria. Red dragon fruit peel contains potentially antibacterial compounds such as flavonoid, alkaloid, saponin, tannin dan terpenoid. The purpose of this study is to determine the antibacterial activity of ethanolic extract of red dragon fruit peel against staphylococcus aureus ATCC 25923.

Method: The method used is true experimental post test only control group design. The activity of ethanol extract of red dragon fruit skin was tested in various concentrations (25%, 50%, 75%, and 100%) against staphylococcus aureus ATCC 25923 by measuring the inhibition zone diameter and minimum inhibitory concentration (MIC).

Result: The results of this study show that red dragon fruit peel ethanolic extract has antibacterial activity against staphylococcus aureus ATCC 25923 ith the average diameter of inhibition zone ranging from 8.25 mm – 12.5 mm. The MIC is obtained at concentration of 25%. Kruskal-Wallis test found a significant comparison with a value of p = 0,000 (p value <0.05). Mann-Whitney test showed that there were significant differences between each concentration, except the concentration of 50% did not have a significant difference with a concentration of 75%.

Conclusion: The minimum inhibitory concentration (MIC) is produced by extract at a concentration of 25% and the best inhibitory zone produced by 100% concentration of red dragon peel ethanol extract.

References

Setiawati A. Peningkatan Resistensi Kultur Bakteri staphylococcus aureus terhadap Amoxicillin Menggunakan Metode Adaptif Gradual. Jurnal Farmasi Indonesia. 2015;7(3):190-194.

WHO (World Health Organization). WHO Global Strategy for Containment of Antimicrobial Resistance. 2001.

Wirahjasa N. Panji AS. Pengelolaan Infeksi Akibat methicillin resistent staphylococcus aureus. E-Medika Udayana. 2012;2(3):135-143.

Hardiana RW. Efektivitas Ekstrak Kulit Buah Naga Merah (hylocereus polyrhizus) terhadap Pertumbuhan streptococcus mutans dan candida albicans (In Vitro) (Skripsi). Jember: Fakultas Kedokteran Gigi Universitas Jember. 2016.

Wahdaningsih S, Untari EK, Fauziah Y. Antibakteri Fraksi n-Heksana Kulit hylocereus polyrhizus Terhadap staphylococcus epidermidis dan propionibacterium acnes. Pharm Sci Res. 2014;1(3):180-193.

Tammi A. Perbandingan Daya Hambat Ekstrak Daun Salam (syzygium polyanthum [Wight.] Walp.) Terhadap Pertumbuhan Bakteri staphylococcus aureus dan Escherichia coli secara In Vitro (Skripsi). Bandar Lampung: Fakultas Kedokteran Universitas Lampung. 2016.

Zakaria S, Syah NH, Firdaus YM. Aktivitas Antibakteri Dari Fraksi Artocarpus Integer (Thunb.) Merr. Dengan Metode Difusi Agar. Jurnal Industri Hasil Perkebunan. 2017;12(2):1-6.

Jawetz E, Melnick JL, Adelberg EA, editor. Mikrobiologi Kedokteran: Edisi XXII. Jakarta: Salemba Medika. 2001.

Suhartati R, Roziqin DA. Aktivitas Antibakteri Ekstrak Etanol Kulit Buah Naga Merah (hylocereus polyrhizus) Terhadap Bakteri streptococcus pyogenes. Jurnal Kesehatan Bakti Tunas Husada. 2017;17(2):513-518.

Tortora GJ, Funke GR, Case CL. Microbiology: edisi ke-9. San Francisco: Pearson Education. 2007.

Cushnie TPT, Cushnie B, Lamb AJ. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents. 2014;44:377-386.

Tandio D, Manuaba A. Safety Procedure for Biosafety and Controlling a Communicable Disease: Streptococcus Suis. Bali Medical Journal. 2016;5(2): 260-262. DOI:10.15562/bmj.v5i2.220.


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