Research Article

The effect of young pepaya leaf ethanol extract (Carica papaya L.) with concentration variants on the growth of pathogenic Streptococcus pyogenes bacteria causing pharyngitis through in vitro testing

I Gede Krisna Putra Yasa, Agung Nova Mahendra , Bagus Komang Satriyasa

I Gede Krisna Putra Yasa
Medical Education Program, Faculty of Medicine, Udayana University, Denpasar, Indonesia

Agung Nova Mahendra
Department of Pharmacology and Therapeutics, Faculty of Medicine, Udayana University, Denpasar, Indonesia. Email: novamahendra@unud.ac.id

Bagus Komang Satriyasa
Department of Pharmacology and Therapeutics, Faculty of Medicine, Udayana University, Denpasar, Indonesia
Online First: August 01, 2019 | Cite this Article
Putra Yasa, I., Mahendra, A., Satriyasa, B. 2019. The effect of young pepaya leaf ethanol extract (Carica papaya L.) with concentration variants on the growth of pathogenic Streptococcus pyogenes bacteria causing pharyngitis through in vitro testing. Intisari Sains Medis 10(2). DOI:10.15562/ism.v10i2.402


Background: Pharyngitis infection issues by Streptococcus pyogenes bacteria have been increasing worldwide, and antibiotic resistance to the bacterium treatment also continues to escalate. Young papaya leaves (Carica papaya L.) have a bactericidal and bacterostatic effect (include papain, flavonoids, alkaloids (karpain alkaloids), saponins, glycosides, phenols (tacophenol), and tannins) that can be an option for treatment of pharyngitis infections other than drugs. This study aims to determine the effectiveness of ethanol extract of young papaya leaves (Carica papaya L.) in inhibiting the growth of Streptococcus pyogenes bacteria through in vitro testing.

Methods: This study was a true-post experimental study using control group design. The sample was divided into 2 groups: positive and negative control groups as well as treatment groups with concentration variants of 25%, 50%, 75%, and 100%. Data were analyzed using SPSS version 17 software for windows.

Results: The result obtained from this study showed that papaya leaf extract (Carica papaya L.) was proven to significantly inhibit the growth of Streptococcus pyogenes bacteria at concentrations of 25% to 100% with an average diameter of the inhibition zone of 6.4 mm to 7.4 mm (P<0.05).

Conclusion: This evidences that the ethanol extract of young papaya leaves (Carica papaya L.) with various concentrations does exhibit an influence on the growth of Streptococcus pyogenes bacteria.

References

Anjos LMM, Marcondes MB, Lima MF, Mondelli LA, Okoshi MP. Streptococcal Acut Faringitis. Revisita da Sociedade Brasileira de Meddicina Tropical. 2014; 47(4):409-413.

Nizet V, Arnold JC. Streptococcus pyogenes (Group A Streptococcus). Etiologic Agents of Infectious Diseases. Section A Bakteri. 2016; 698-707.

Dipiro JT, Talbert RL, Yee GC, Matzke GR. Wells BG. Posey LM. Pharmacotherapy, A Pathophysiologic Approach 7th Edition. United States of America : The McGraw- Hill Companies, Inc. 2008.

Umar F, Zardania E, Nirwani R, Purnama, Istiqomah SN, Masrul, Rahim, et al. Pharmaceutical Care Untuk Penyakit Infeksi Saluran Pernapasan. Departemen Kesehatan Republik Indonesia. 2005

Cunningham MW. Pathogenesis of Group A Streptococcal Infection. Clin Microbiol Rev. 2000; 13(3):470-511.

Katerov V. Streptococcal Cell Surface Protein: Structure and Gene Characterization. Institute of Infectious Diseases and Medical Microbiology, Lund University. 2002; 2(3):1-34.

Todar K. Todar’s Online Textbook of Bacteriology: Streptococcus pyogenes, Universitas of Wisconsin-Madison Departement of Bacteriology. 2002.

Pratiwi EW, Praharani D, Arina YMD. Daya hambat ekstrak daun pepaya (Carica papaya L.) terhadap adehsi bakteri Porphyromonas gingivalis pada neutrofil. E-Jurnal Pustaka Kesehatan. 2015; 3(2):193-198

Rahayu S, Tjitraresmi A. Tanaman Pepaya (Carica papaya L.) dan Manfaatnya Dalam Pengobatan. Farmaka. 2016; 4(1):1-17

Suresh K, Deepa P, Harisaranraj R, Vaira AV. Antimicrobial and Phytochemical Investigation of the Leaves of Carica papaya L., Cynodon dactylon (L.) Pers., Euphorbia hirta L., Melia azedarach L. and Psidium guajava L. Ethnobotanical Leaflets. 2008; 12:1184-91.

Tuntun,M. Uji efektivitas daun pepaya (Carica papaya L) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kesehatan. 2016: 7(3):497-502.

Sudarwati, TPL. Aktivitas Antibakteri Daun Pepaya (Carica papaya) Menggunakan Pelarut Etanol Terhadap Bakteri Bacillus subtilis. Journal of Pharmacy and Science. 2018: 3(2):2527-6328.

Soesanto L. Pengantar Pengendalian Hayati Penyakit Tanaman, Suplemen ke Gulma dan Nematoda. Rajawali Pers. 2008: 573.

Arulanantham R. Alternative Culture Media for Fungal Growth Using Different Formulation of Protein Sourcs. J. Nat. Prod. Plant Resour. 2012; 2(6):697-700.

Sugito, Suwandi, Edy. Efektifitas Ekstrak Ethanol Daun Pepaya (Carica papaya L) terhadap Pertumbuhan Bakteri Escherichia coli dengan Metode Difusi. Jurnal Laboratorium Khatulistiwa. 2017; 1(1):21-25.

Pelezar JR, Michael J. Dasar – dasar Mikrobiologi 2. Jakarta: Penerbit Universitas Indonesia (UI Press); 1988.

Yasni,S. Teknologi Pengolahan dan Pemanfaatan Produk Ekstrak Rempah. Bogor: PT.Penerbit IPB Pess; 2013.

Cowan MM. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews. 1999: 12: 564 – 582.

Nuria MC, Faizatun A, Sumantri. Uji Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha cuircas L) terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Jurnal Ilmu – Ilmu Pertanian. 2009: 5: 26 – 37.

Pietta P, Simonetti P. Antioxidant Food Supplement in Human Health: Dietary Flavonoid and Interaction with Physiologic Antioxidants. London: Academic Press . 1999.

Lestari AAW, Sukrama IDM, Nurmansyah D. The earthworm (Lumbricus rubellus) extract decreased amino transaminase enzyme level and number of bacterial colonu in male wistar rats infected with Salmonella thypimurium. Biomed Pharmacol. J. 2019; 12(1):325-332


No Supplementary Material available for this article.
Article Views      : 28
PDF Downloads : 22