Research Article

Aktivitas secara in vitro dan in vivo kombinasi ekstrak daun kelor (Moringa oleifera lam.) dan pegagan (Centella asiatica (l.). Urb.) sebagai gel anti jerawat

Ninik Setya Hastuti , Shelly Taurhesia, Agung Eru Wibowo

Ninik Setya Hastuti
Program Magister Ilmu Kefarmasian, Fakultas Farmasi, Universitas Pancasila, Jakarta. Email: drniniksetya@gmail.com

Shelly Taurhesia
Head of Tech Reg & QA Amway Indonesia

Agung Eru Wibowo
Pusat Penelitian Ilmu Pengetahuan dan Teknologi Indonesia
Online First: December 01, 2019 | Cite this Article
Hastuti, N., Taurhesia, S., Wibowo, A. 2019. Aktivitas secara in vitro dan in vivo kombinasi ekstrak daun kelor (Moringa oleifera lam.) dan pegagan (Centella asiatica (l.). Urb.) sebagai gel anti jerawat. Intisari Sains Medis 10(3). DOI:10.15562/ism.v10i3.351


Background: Acne is a skin problem that is common in everyday life. Expressions of acne can cause psychological problems because of appearance. Moringa leaf extract and herbs contain alkaloid compounds, flavonoids, saponins, tannins and phenolics that can be used as antibacterial, reduce inflammation, reduce sebum glands and moisturize the skin. The purpose of this study was to conduct an in vitro and in vivo analysis of gel activity in combination of Moringa Oleifera L. leaf extract and Centella asiatica (L.). Urb.) Herb or KEKP gel as an anti-acne gel.

Method: Activities carried out in vitro with an antibacterial effect on P. acnes, in vivo are carried out by clinical observation and measurement of sebum secretion. The gel contains the same combination of extracts. Twelve volunteers who met the inclusion and exclusion criteria followed the in vivo test to determine the safety and benefits of KEKP gel.

Results: At a concentration of 0.78% dilution shows a diameter of 10.4 mm inhibitory power. Medikline as a positive control gives better results compared to KEKP gel with a minimum inhibition diameter of 16.6 mm. Clinical improvement was assessed by reduced signs of inflammation, the number of papules, pustules, nodules and changes in sebum levels measured using a skin analysis tool.

Conclusion: The results showed that KEKP antifungal gel invitro had antibacterial activity, and in vivo showed real clinical differences

References

Eshtiaghi MN, Kuldiloke J. Formulation of anti acne cream containing natural antimicroba. International Research Journal Of Pharmacy. 2013;4(11):20-25.

Webster GF. Acne Vulgaris. BMJ. 2002;325(7362):475–9.

Well D. Acne vulgaris: A review of causes and treatment options. Nurse Pract. 2013;38(10):22-31

Strauss JS, Krowchuck DP, Leyden JJ, et al. Guidelines of care for acne vulgaris management. J Am Acad Dermatol. 2007;56(4):651-63

Sinha P, Srivastava S, Mishra N,et al. New perspectives on antiacne plant drugs : contribution to modern therapeutics. Biomed Res Int. 2014;2014:301304.

Ndhlala AR, Mulaudzi R, Ncube B, et al. Antioxidant, Antimicrobial and Phytochemical Variations in Thirteen Moringa oleifera Lam. Cultivars. Molecules. 2014;19(7): 10480-94

Leone A, Spada A, Battezzati A, et al.. Cultivation, Genetic, Ethnopharmacology, Phytochemistry and Pharmacology of Moringa oleifera Leaves. Int J Mol Sci. 2015; 16(6):12791–835

Abalaka ME, Daniyan SY, Oyeleke SB, et al. The Antibacterial Evaluation of Moringa Oleifera Leaf Extracts on Selected Bacterial Pathogens. Journal of Microbiology Research. 2012;2(2):1-4

Marrufo T, Nazzaro F, Mancini E, et al. Chemical Composition and Biological Activity of the Essential Oil from Leaves of Moringa oleifera Lam. Cultivated in Mozambique. Molecules. 2013;18(9):10989-11000.

Ali A, Akhtar N, Khan. MS, et al. Effect of Moringa oleifera on undesireble skin sebum secretions of sebaceous glands observed during winter season in humans. Biomedical Research. 2013;24(1):127-130.

Samy RP, Chow VTK. Antimicrobial and Phytochemical Analysis of Centella asiatica (L.). Nature Precdings. 2011:1-6

Dash BK, Faruquee HM, Biswas SK, et al. Antibacterial and Antifungal Activities of Several Extracts of Centella asiatica L. against Some Human Pathogenic Microbes. Life Sciences and Medicine Research. 2011; LSMR-35

Oyedeji OA, Afolayan AJ. Chemical compotion and antibacterial activity of the Essential oil of centella asiatica. Growing in South Afrika. Journal Pharmaceutical Biology. 2005;(43):249–252.

Bylka W, Znajdek-Awiżeń P, Studzińska-Sroka E, Brzezińska M. Centella asiatica in cosmetology. Postep Derm Alergol. 2013;30(1):46-49

Chomnawang MT, Surassmo S, Nukoolkarn V, Gritsanapan W. Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria. Journal of Ethnopharmacology. 2005;101(1-3):330–3.

Rismana E, Rosidah I, Prasetyawan Y, Bunga O, Erna Y. Efektivitas Khasiat Pengobatan Luka Bakar Sediaan Gel Mengandung Fraksi Ekstrak Pegagan Berdasarkan Analisis Hidroksiprolin Dan Histopatologi Pada Kulit Kelinci. Bul. Penelit. Kesehat, 2013;41(1):45–60.

Wasitaatmadja SM. Manifestasi klinis, klasifikasi, dan stadium akne. National Symposium and Workshop in Cosmetic Dermatology. Acne: New concept and challenges. Jakarta. 2010


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