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The effect of red-cabbage (Brassica oleracea L. var. capitata f. rubra) ethanolic extract in cream preparation on dermis-elastic fibre thickness in male Wistar rats after ultraviolet-B rays exposure

Abstract

Background: Ultraviolet B (UV-B) rays exposure is a leading cause of sunburn, skin dryness and skin aging by influencing the dermal elastic fibers. This photoaging effect was caused by damage of fibrillin-1 as a microfibrillar network. This study aims to evaluate the effect of red-cabbage (Brassica oleracea L. var. capitata f. rubra) ethanolic extract in cream preparation on dermis-elastic fibers thickness in male Wistar rats after UV-B exposure.

Methods: A randomized post-test only control group design was conducted among 25 male Wistar rats during 2 weeks. They were divided into 5 groups as follows: control group (P1), basic cream (P2), 5% ethanolic extract of red cabbage cream (P3), 10% ethanolic extract of red cabbage cream (P4), and 20% ethanolic extract of red cabbage cream (P5). The UV-B dosage used are 325 mJ/cm2 among groups prior to study. The dermis-elastic fiber thickness was assessed by Image Raster software. Statistical analysis was carried out using SPSS version 20 in measuring normality test, mean, and ANOVA

Results: There was a statistically significant difference of dermal-elastic fibers thickness among groups (P < 0.05). The mean of dermis-elastic fiber thickness was 6.125±0.376 µm in P5 group, followed by 4.075±0.266 µm in P4 group, and 3.114±0.290 µm, 2.109±0.420 µm, and 1.907±0.454 µm in P3, P2, and P1 group respectively.

Conclusion: Ethanolic extract of red-cabbage in a different dose of cream preparation have a statistically significant difference on the dermis-elastic fibers thickness after UV-B exposure

References

  1. Borumand M, Sibilla S. Daily consumption of the collagen supplement reduces visible signs of aging. Clin Interv Aging. 2014; 9:1747-1758.
  2. Kielty CM, Sherratt MJ, Shuttleworth CA. Elastic fibres. J Cell Sci. 2002; 115(Pt 14):2817-28.
  3. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med. 1997; 337(20):1419-28.
  4. Amaro-Ortiz A, Yan B, D’Orazio J. Ultraviolet radiation, aging and the skin: prevention of damage by topical cAMP manipulation. Molecules. 2014; 19(5):6202-6219.
  5. Poljsak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract. 2012; 10:1155-1352.
  6. Langton AK, Sherratt MJ, Griffiths CEM, Watson RE. A new wrinkle on old skin: the role of elastic fibres in skin ageing. Int J Cosmet Sci. 2010; 32(5):330-9.
  7. Ahmed MF, Rao AS, Ahemad SR, Ibrahim M. Phytochemical Studies and Antioxidant Activities of Brassica oleracea L. var. Capitata. Int J Pharm Pharm Sci, Vol 4, 2012; 3:374-378.
  8. Sotelo T, Cartea ME, Velasco P, Soengas P. Identification of Antioxidant Capacity Related QTLs in Brassica olerace. PLoS One. 2014; 9(9): e107290.
  9. Imokawa G, Ishida K. Biological mechanisms underlying the ultraviolet radiation-induced formation of skin wrinkling and sagging I: reduced skin elasticity, highly associated with enhanced dermal elastase activity, triggers wrinkling and sagging. Int J Mol Sci. 2015; 16(4):7753-75
  10. Bhattacharyya TK, Thomas JR. Histomorphologic changes in aging skin observations in the CBA mouse model. Arch Facial Plast Surg. 2004; 6(1):21-5.
  11. Krismayogi GA, Ratnayanti IGAD, Linawati NM, Wiryawan IGNS, Sugiritama IW, Wahyuniari IAI, Arijana IGKN. Purple Cabbage Extract Cream Effect on Erythema Score of Male Wistar Rats Back Skin Exposed to UV-B Radiation. Biomedical and Pharmacology Journal. 2018; 11(1):345-351

How to Cite

Bt Abdul Rauap, I. S., Sugiritama, I. W., Linawati, N. M., & Arijana, I. G. K. (2018). The effect of red-cabbage (Brassica oleracea L. var. capitata f. rubra) ethanolic extract in cream preparation on dermis-elastic fibre thickness in male Wistar rats after ultraviolet-B rays exposure. Intisari Sains Medis, 9(3). https://doi.org/10.15562/ism.v9i3.286

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Intan Syahirah Bt Abdul Rauap
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I Wayan Sugiritama
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Ni Made Linawati
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I Gusti Kamasan Arijana
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