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Ethanolic extract cream of red cabbage (Brassica oleracea L. var. capitata f.rubra) on the epidermal thickness in Wistar rats (Rattus norvegicus) after ultraviolet-B exposure

Abstract

Background: UV exposure is related to oxidative stress and also as an essential factor in skin photoaging. Several antioxidants are widely used in preventing skin photoaging by influencing epidermal skin layer. High concentrations of antioxidant properties in red cabbage are known to have these effects. This study aims to determine the role of ethanolic extract cream of red cabbage on the epidermal thickness in Wistar rats after UV-B exposure.

Methods: An experimental study was carried out among 25 Wistar rats which divided into 5 groups as follows: P1 as control (only exposed to UB-B), P2 (placebo and UV-B), P3 (5% ethanolic extract cream and UV-B), P4 (10% ethanolic extract cream and UV-B), and P5 (20% ethanolic extract cream and UVB). The UV-B dosage applied for each group was 65 mJ/cm2. The thickness of epidermis is calculated by direct measurement using Image Raster software from basal membrane to stratum corneum. The statistical analyses were performed using SPSS ver. 20.0.

Results: There was a significantly different in the epidermal thickness among groups (P < 0.05). The mean of epidermal thickness in the control group (P1) was 36.00±1.08 µm, followed by 27.66±0.87 µm in the treatment group I (P2), 26.76±1.02 µm in the treatment group II (P3), 24.94±1.25 µm in the treatment group III (P4), and 20.24±1.47 µm in the treatment group IV (P5).

Conclusion: The ethanolic extract cream of red cabbage (Brassica oleracea L.Var. capitata f. rubra) in different doses have a statistically significant impact on the epidermal thickness in Wistar rats after UV-B exposure.

References

  1. Mukherjee S, Date A, Patravale V, Korting HC, Roeder A, Weindl G. Retinoids in the treatment of skin aging: an overview of clinical efficacy and safety. Clin Interv Aging. 2006:1(4) 327–348.
  2. Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermatoendocrinol. 2012; 4(3):308–319.
  3. Poljšak B, Dahmane R. Free radicals and extrinsic skin aging. Dermatol Res Pract. 2012; 2012:135206.
  4. Binic I, Lazarevic V, Ljubenovic M, Mojsa J, Sokolovic D. Skin Ageing: Natural Weapons and Strategies. Evid Based Complement Alternat Med. 2013; 2013; 827248.
  5. Helfrich YR, Sachs DL, Voorhees JJ. Overview of skin aging and photoaging. Dermatol Nurs. 2008; 20(3):177-83.
  6. Telang PS. Vitamin C in dermatology. Indian Dermatol Online J. 2013; 4(2): 143–146.
  7. Draghici GA, Lupu MA, Borozan AB, NIca D, Alda S, Alda L et al. Red cabbage, millennium’s functional food. Journal of Horticulture, Forestry, and Biotechnology. 2013; 17(4), 52- 55.
  8. Anahi B, Norena CPZ. Encapsulation of Red Cabbage (Brassica oleracea L. var.capitata L. f. rubra) Anthocyanins by Spray Drying using Different Encapsulating Agents. Brazilian Archives of Biology and Technology. 2015; 56(6): 944-952
  9. Krutmann J, Bouloc A, Sore G, Bernard BA, Passeron T. The skin aging exposome. J Dermatol Sci. 2017; 85(3):152-161.
  10. Chauhan ES, Tiwari A, Singh A. Phytochemical screening of red cabbage (Brassica oleracea) powder and juice - A comparative study. Journal of Medicinal Plants Studies. 2016; 4(5): 196-199.
  11. Hassan SMA, Hussein AJ, Saeed AK. Role of Green Tea in Reducing Epidermal Thickness upon Ultraviolet Light-B Injury in BALB/c Rat. Advances in Biology. 2015; 2015; 890632.
  12. Kim HK. Garlic Supplementation Ameliorates UV-Induced Photoaging in Hairless Mice by Regulating Antioxidative Activity and MMPs Expression. Molecules. 2016 Jan 8;21(1):70.

How to Cite

Rajappan, Y. V., Arijana, I. G. K., Sugiritama, I. W., & Ratnayanti, I. D. (2018). Ethanolic extract cream of red cabbage (Brassica oleracea L. var. capitata f.rubra) on the epidermal thickness in Wistar rats (Rattus norvegicus) after ultraviolet-B exposure. Intisari Sains Medis, 9(3). https://doi.org/10.15562/ism.v9i3.279

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Yogarani V.C Rajappan
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I Gusti Kamasan Arijana
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I Wayan Sugiritama
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IGA Dewi Ratnayanti
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