Skip to main content Skip to main navigation menu Skip to site footer

Kondisi optimal PCR untuk mendeteksi gen FimH isolat klinis Escherichia coli penyebab infeksi saluran kemih


Background: Urinary tract infection is one of the most common infectious diseases and the second most common infection after respiratory tract infection. Bacteria are the most common cause of urinary tract infections in more than 95% of cases. Escherichia coli is the most common bacteria that found in 80-90% of cases of urinary tract infection. The critical virulence factor of Escherichia coli is the type 1 fimbriae with the adhesion subunit, fimH, plays an essential role in the pathogenesis of urinary tract infection.

Aim: The research aims to obtain the optimal condition of PCR in detecting the fimH gene with modification of annealing temperature in the PCR process.

Method: This study used a sample of fimH DNA genes from clinical isolates of E. coli causing UTI. The second PCR used an annealing temperature of 57°C and a third PCR using an annealing temperature of 64°C. The electrophoresis results are then viewed with an ultraviolet transilluminator.

Result: The optimization finding showed that the annealing temperature of 52 °C, 57 ° C, and 64°C could detect the fimH gene where the higher the temperature used by Tm, the less the specificity produced.

Conclusion: The optimum annealing temperature of PCR to identify the fimH gene from E.coli causes the UTI is in the temperature of 64°C with better specificity than of 52°C. The annealing temperature of 64°C indicates the lowest specificity.


  1. Hotchandani R and Aggarwal KK. Urinary Tract Infections in Women. Ind J Clin Practice. 2012;187-192.
  2. Iranpour D, Hassanpour M, Ansari H, Tajbakhsh S, Khamisipour G, Najafi A. Phylogenetic groups of Escherichia coli strains from patients with urinary tract infection in Iran based on the new Clermont phylotyping method. Biomed Res Int. Hindawi Publishing Corporation. 2015: 5–11.
  3. World Health Organization. Cauda UTI - ECCMID Dec 2011DEF.ppt. (Akses: 30 April 2018).
  4. Daoud Z, Afif C. Escherichia coli Isolated from Urinary Tract Infections of Lebanese Patients between 2000 and 2009: Epidemiology and Profiles of Resistance. Chemother Res Pract. 2011; 1–6.
  5. Rogers EMC, Peterson ND, Hilbert DW. Uropathogenic Escherichia coli: the pre-eminent urinary tract infection pathogen; 2011: 1-66 p.
  6. Minardi D, d’Anzeo G, Cantoro D, Conti A, Muzzonigro G. Urinary tract infections in women: etiology and treatment options. Int J Gen Med. 2011;4:333–43.
  7. Tabasi M, Reza M, Karam A, Habibi M. Phenotypic Assays to Determine Virulence Factors of Uropathogenic Escherichia coli ( UPEC ) Isolates and their Correlation with Antibiotic Resistance Pattern. Osong Public Heal Res Perspect. Elsevier Korea LLC; 2015;6(4):261–8.
  8. Tiba MR, Yano T, Leite DDS. Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis. Rev Inst Med Trop Sao Paulo. 2008;50(5):255–60.
  9. Slavchev G, Pisareva E, Markova N. Virulence of Uropathogenic Escherichia Coli. J Cult Collect. 2008;6:3–9.
  10. Hojati Z, Molaie R, Gholipour A. The FimH Gene in Uropathogenic Escherichiacoli Strains Isolated From Patient With Urinary Tract Infection. Jundishapur J Microbiol [Internet]. 2015;8(2):12–5.

How to Cite

Zharaswati, P., Budayanti, N. N. S., & Fatmawati, N. N. D. (2019). Kondisi optimal PCR untuk mendeteksi gen FimH isolat klinis Escherichia coli penyebab infeksi saluran kemih. Intisari Sains Medis, 10(2).




Search Panel

Prisela Zharaswati
Google Scholar
ISM Journal

Ni Nyoman Sri Budayanti
Google Scholar
ISM Journal

Ni Nengah Dwi Fatmawati
Google Scholar
ISM Journal