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Pengaruh filtrat fermentasi nira kelapa (Cocos nucifera L.), nira jaka (Arenga pinnata), nira ental (Borassus flabellifer), dan ubi jalar ungu (Ipomoea batatas L.) terhadap ketebalan epidermis kulit tikus Wistar yang dipapar sinar UVB

Abstract

Background: Skin aging is influenced by various factors, one of which is UV rays. Photoaging can cause thinning of the epidermis. Fermented products are known to inhibit the reduction in epidermis thickness. This study aims to identify the effect of fermented filtrate of coconut palm wine, jaka palm wine, ental palm wine and purple sweet potato on the thickness of the skin epidermis of Wistar rats exposed to UVB rays.

Methods: Post test only control group is the design of this research. The samples used in this research were 51 Wistar rats divided into 17 groups with treatment for one month. The control group was exposed to UVB 840 mJ and given a smear of aquabidest. The treatment group was given UVB 840 mJ and fermented filtrate of coconut palm wine, jaka palm wine, ental palm wine and purple sweet potato with concentrations of 25%, 50%, 75% and 100%.

Results: The differences of epidermis thickness were found significant (p<0.05) between the control group (mean thickness 37.05mm) and the coconut palm wine fermentation filtrate treatment group with concentrations of 50% (mean thickness 48.94mm), 75% (mean thickness 52.94mm), palm wine fermentation filtrate with a concentration of 50% (average thickness 47.83mm) and purple sweet potato with a concentration of 25% (average thickness 51.73mm).

Conclusion: Fermented filtrate of coconut palm wine 50% and 75%, jaka palm wine 50% and purple sweet potato 25% inhibits the decrease in the thickness of the skin epidermis of Wistar rats exposed to UVB light.

 

Latar Belakang: Penuaan kulit dipengaruhi oleh berbagai faktor salah satunya adalah sinar UV. Photoaging dapat menyebabkan penipisan epidermis. Produk fermentasi diketahui dapat menghambat penurunan ketebalan epidermis. Penelitian ini bertujuan untuk mengidentifikasi pengaruh filtrat fermentasi nira kelapa, nira jaka, nira ental dan ubi jalar ungu terhadap ketebalan epidermis kulit tikus wistar yang dipapar sinar UVB.

Metode: Post test only control group merupakan desain penelitian ini. Sampel yang digunakan dalam penelitian ini adalah 51 ekor tikus wistar yang dibagi menjadi 17 kelompok dengan perlakuan selama satu bulan. Kelompok kontrol dipapar UVB 840 mJ dan diberikan olesan aquabides. Kelompok perlakuan diberikan UVB 840 mJ dan filtrat fermentasi nira kelapa, nira jaka, nira ental dan ubi jalar ungu dengan konsentrasi 25%, 50%, 75%, dan 100%.

Hasil Penelitian: Perbedaan rerata ketebalan epidermis yang signifikan (sig.< 0,05) antara kelompok kontrol (rerata ketebalan 37,05 mm) terhadap kelompok perlakuan filtrat fermentasi nira kelapa dengan konsentrasi 50% (rerata ketebalan 48,94 mm), 75% (rerata ketebalan 52,94 mm), filtrat fermentasi nira jaka dengan konsentrasi 50% (rerata ketebalan 47,83 mm) dan ubi jalar ungu dengan konsentrasi 25% (rerata ketebalan 51,73 mm).

Kesimpulan: Filtrat fermentasi nira kelapa 50% dan 75%, nira jaka 50%, dan fermentasi ubi jalar ungu 25% menghambat penurunan ketebalan epidermis kulit tikus Wistar yang dipapar sinar UVB

References

  1. Xie C, Jin J, Lv X, Tao J, Wang R, Miao D. Anti-aging effect of transplanted amniotic membrane mesenchymal stem cells in a premature aging model of bmi-1 deficiency. Sci Rep. 2015;5(August):1–18.
  2. Mariati DE, Sudigdoadi S, Lesmana R, Khairani AF, Gunadi JW, Tarawan VM, et al. Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1. Indones Biomed J. 2021;13(1):84–90.
  3. Wang Z, Man MQ, Li T, Elias PM, Mauro TM. Aging-associated alterations in epidermal function and their clinical significance. Aging (Albany NY). 2020;12(6):5551–65.
  4. Kohl E, Steinbauer J, Landthaler M, Szeimies RM. Skin ageing. J Eur Acad Dermatology Venereol. 2011;25(8):873–84.
  5. Coelho SG, Choi W, Brenner M, Miyamura Y, Yamaguchi Y, Wolber R, et al. Short- and long-term effects of UV radiation on the pigmentation of human skin. J Investig dermatology Symp Proc. 2009;14(1):32–5.
  6. Gilchrest BA. Photoaging. J Invest Dermatol. 2013;133(E1):E2-6.
  7. Indrakusuma AABP, Wahyuni LPS, Wiguna IGWW, Devy AAT, Sasmana IGAP, Indrayani AW. Potential effect of secondary metabolites in Persea americana seeds as an a-amylase inhibitor on type 2 diabetes mellitus. Intisari Sains Medis. 2021;12(3):886.
  8. Yong CC, Yoon Y, Yoo HS, Oh S. Effect of Lactobacillus Fermentation on the Anti-Inflammatory Potential of Turmeric. J Microbiol Biotechnol. 2019;29(10):1561–9.
  9. Hur SJ, Lee SY, Kim Y-C, Choi I, Kim G-B. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014;160:346–56.
  10. Federer WT. Randomization and Sample Size in Experimentation. In: the Food and Drug Administration Statistics Seminar. 1966. p. 15.
  11. Lavker RM. Structural alterations in exposed and unexposed aged skin. J Invest Dermatol. 1979;73(1):59–66.
  12. Gilchrest BA, Blog FB, Szabo G. Effects of aging and chronic sun exposure on melanocytes in human skin. J Invest Dermatol. 1979;73(2):141–3.
  13. Russell-Goldman E, Murphy GF. The Pathobiology of Skin Aging: New Insights into an Old Dilemma. Am J Pathol. 2020;190(7):1356–69.
  14. Lee H, Hong Y, Kim M. Structural and Functional Changes and Possible Molecular Mechanisms in Aged Skin. Int J Mol Sci. 2021;22(22).
  15. Sasmana IGAP, Wiranata S, Yogananda KC, Wihandani DM, Supadmanaba IGP. Clinicopathological and Prognostic Significance of SRY-Box Transcription Factor 2 ( SOX2 ) Overexpression in Central Nervous System Tumor : A Meta-Analysis. Bali Med J. 2023;12(2):1733–9.
  16. Gede I, Laksana Jagadhita A, Ayu G, Ratnayanti D, Sugiritama W, Kamasan G, et al. Analisis Fitokimia Nira Dan Nira Kelapa (Cocos Nucifera L.). J Med Udayana. 2022;11(2):65–9. Available from: https://ojs.unud.ac.id/index.php/eum
  17. Ayu ID, Widiari, Eka IG, Ratnayanti D, Sugiritama IW, Gusti I, Arijana KN. Analisis Fitokimia Nira Aren dan Nira Aren (Arenga pinnata (Wurmb) Merr.). J Med Udayana. 2021;10(6):18–22.
  18. Oki T, Osame M, Masuda M, Kobayashi M, Furuta S, Nishiba Y, et al. Simple and rapid spectrophotometric method for selecting purple-fleshed sweet potato cultivars with a high radical-scavenging activity. Breed Sci. 2003;53(2):101–7.
  19. Valadez-Carmona L, Cortez-García RM, Plazola-Jacinto CP, Necoechea-Mondragón H, Ortiz-Moreno A. Effect of microwave drying and oven drying on the water activity, color, phenolic compounds content and antioxidant activity of coconut husk (Cocos nucifera L.). J Food Sci Technol. 2016;53(9):3495–501.
  20. Leyva-López N, Gutierrez-Grijalva EP, Ambriz-Perez DL, Basilio Heredia J. Flavonoids as cytokine modulators: A possible therapy for inflammation-related diseases. Int J Mol Sci. 2016;17(6).
  21. Al-Khayri JM, Sahana GR, Nagella P, Joseph B V., Alessa FM, Al-Mssallem MQ. Flavonoids as Potential Anti-Inflammatory Molecules: A Review. Molecules. 2022;27(9).
  22. Sasmana IGAP, Kusuma IKWA, Dhananjaya IGAD, Devy AAT, Wihandani DM. Pengaruh ekstrak aqueous Clitoria ternatea terhadap kerusakan histologi otak dan gangguan memori pada tikus wistar terinduksi diet tinggi lemak. J Med Udayana. 2023;12(3):78–83.
  23. Dharmawansa KVS, Hoskin DW, Rupasinghe HPV. Chemopreventive Effect of Dietary Anthocyanins against Gastrointestinal Cancers: A Review of Recent Advances and Perspectives. Int J Mol Sci. 2020;21(18).
  24. Khoo HE, Azlan A, Tang ST, Lim SM. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017;61(1). Available from: https://doi.org/10.1080/16546628.2017.1361779
  25. Vidana Gamage GC, Lim YY, Choo WS. Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications. Front Plant Sci. 2021;12(December):1–17.

How to Cite

Pinatih, N. M. M. M. R., Ratnayanti, I. G. A. D. ., Wahyuniari, I. A. I. ., & Linawati, N. M. . (2024). Pengaruh filtrat fermentasi nira kelapa (Cocos nucifera L.), nira jaka (Arenga pinnata), nira ental (Borassus flabellifer), dan ubi jalar ungu (Ipomoea batatas L.) terhadap ketebalan epidermis kulit tikus Wistar yang dipapar sinar UVB. Intisari Sains Medis, 15(1), 92–96. https://doi.org/10.15562/ism.v15i1.1932

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Ni Made Meisya Maha Rani Pinatih
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I Gusti Ayu Dewi Ratnayanti
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Ida Ayu Ika Wahyuniari
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Ni Made Linawati
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