Skip to main content Skip to main navigation menu Skip to site footer

Purple Sweet Potato (Ipomea batatas L.) Cream Extract Increase Superoxide Dismutase (SOD) - Malondialdehyde (MDA) Ratio and Higher Collagen Expression in Rats (Rattus novergicus) Exposed to Ultraviolet B

  • Marvin Giantoro ,
  • Ketut Kwartantaya Winaya ,
  • Ni Made Dwi Puspawati ,

Abstract

Introduction: Aging can occur in all parts of the body. Superoxide dismutase (SOD) enzyme is an enzyme that interacts with collagen, especially in types I and IV. UVB rays can trigger the presence of free radicals and lipid peroxidation, which form malondialdehyde (MDA) compounds. This study aimed to determine the effect of cream application of purple sweet potato (Ipomoea batatas L.) extract on increasing the ratio of SOD to MDA levels and increasing collagen in rats exposed to UVB.

Methods: This was experimental, using a post-test only with a control group design. The irradiation was carried out for 4 weeks with a total radiation dose of 840 mJ/cm2. Purple sweet potato cream concentration was 4%, 8%, and 16%. Rat blood was taken to check the ratio of SOD/MDA and collagen levels using the ELISA kit and Sirius red staining. This study used descriptive analysis and ANOVA using SPSS.25.sav

Results: The highest ratio of SOD/MDA levels was found in the treatment group with an 8% purple sweet potato extract concentration of 3.346 ± 0.113. In the collagen expression, the highest percentage was found at 8% levels of 83,290 ± 1,885%. There was a significant mean difference between the treatment and control groups, with the treatment group having a higher result than the control group (p<0.001).

Conclusion: The administration of purple sweet potato cream extract could inhibit aging by increasing the ratio of SOD/MDA levels and collagen expression.

References

  1. Yousef H, Alhajj M, Sharma S. Anatomy, Skin (Integument), Epidermis. In Treasure Island (FL); 2023.
  2. Pratama GMCT, Hartawan IGNBRM, Indriani IGAT, Yusrika MU, Suryantari SAA, Sudarsa PSS. The potency of Spirulina platensis Extracts as Sunscreen on Ultraviolet B Exposure. J Med Heal. 2020;2(6):205–17. DOI: http://dx.doi.org/10.28932/jmh.v2i6.2484.
  3. Zahruddin A. Penuaan Kulit: Patofisiologi dan Manifestasi Klinis (Skin Aging: Pathophysiology and Clinical Manifestation). Berk Ilmu Kesehat Kulit dan Kelamin. 2018;30(3):208–15.
  4. Ahmad Z, Damayanti. Penuaan Kulit : Patofisiologi dan Manifestasi Klinis. Berk Ilmu Kesehat Kulit dan Kelamin – Period Dermatology Venereol [Internet]. 2018;30(03):208–15.
  5. Zhang S, Duan E. Fighting against Skin Aging: The Way from Bench to Bedside. Cell Transplant. 2018;27(5):729–38. DOI: https://doi.org/10.1177/0963689717725755.
  6. Hwang KA, Yi BR, Choi KC. Molecular mechanisms and in vivo mouse models of skin aging associated with dermal matrix alterations. Lab Anim Res. 2011 Mar;27(1):1–8. DOI: https://doi.org/10.5625/lar.2011.27.1.1.
  7. Tsatsou F, Trakatelli M, Patsatsi A, Kalokasidis K, Sotiriadis D. Extrinsic aging: UV-mediated skin carcinogenesis. Dermatoendocrinol. 2012 Jul;4(3):285–97. DOI: https://doi.org/10.4161/derm.22519.
  8. D’Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013;14(6):12222–48. DOI: https://doi.org/10.3390/ijms140612222.
  9. Pandel R, Poljšak B, Godic A, Dahmane R. Skin Photoaging and the Role of Antioxidants in Its Prevention. ISRN Dermatol. 2013;2013:1–11. DOI: https://doi.org/10.1155/2013/930164.
  10. Lee MJ, Agrahari G, Kim HY, An EJ, Chun KH, Kang H, et al. Extracellular Superoxide Dismutase Prevents Skin Aging by Promoting Collagen Production by Activating AMPK and Nrf2/HO-1 Cascades. J Invest Dermatol. 2021;141(10):2344-2353.e7. DOI: https://doi.org/10.1016/j.jid.2021.02.757.
  11. Herdiani N, Wirjatmadi B, Adriani M. Administration of red rosella’s petals extract (Hibiscus sabdariffa) increase the level of superoxide dismutase (SOD) Wistar rats which induced by repeatedly heated cooking oil. In 2017. DOI: https://doi.org/10.30742/JIKW.V4I2.9.
  12. Putra H, Annis F, Purnomo Y. Efek Ekstrak Etanol Daun Gedi Merah (Abelmoschus Manihot (L.) Medik) Terhadap Kadar Sod Dan Mda Pada Aorta Tikus Model Diabetes Melitus Tipe 2. J Kesehat Islam Islam Heal J. 2020;9(1):1. DOI: http://dx.doi.org/10.33474/jki.v9i1.8861.
  13. Nilla PY, Hussaana A, Chodidjah. Pengaruh Krim Ekstrak Ubi Ungu (Ipomoea Batatas Var Ayumurasaki) terhadap Matriks Metalloproteinase-1 dan Jumlah Kolagen Dermis Tipe I dan Tipe III (Penelitian Eksperimental Pada Mencit BALB/c yang Dipapar Sinar UV-B). J Tunas Med Kedokt Kesehat. 2021;7(1):1–19.
  14. R. DL, L. LNP, V. PNLP, G. WIMA. Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Ubi Jalar Ungu (Ipomoea Batatas (L.) Lam) dengan Metode Ferrous Ion Chelating (FIC). J Farm Udayana. 2014;3(1).
  15. Jawi IM, Yasa IWPS, Mahendra AN. Antihypertensive and Antioxidant Potential of Purple Sweet Potato Tuber Dry Extract in Hypertensive Rats. Bali Med J. 2016;5(2):65. DOI: https://doi.org/10.15562/bmj.v5i2.217.
  16. Dwi Primayanti IDAI, Aman IGM, Bagiada IMA. Ipomoea batatas Syrup Decrease Malondialdehyde and Increase Nitrous Oxide Plasma Levels Among Moderate Smoker Workers at Denpasar. Bali Med J. 2012;1(3):125–30. DOI: https://doi.org/10.15562/bmj.v1i3.28.
  17. Ansel. Pengantar bentuk sediaan farmasi. Glob Shad Africa Neoliberal World Order. 2005;44(2):8–10.
  18. Husna N El, Novita M, Rohaya S. Anthocyanins Content and Antioxidant Activity of Fresh Purple Fleshed Sweet Potato and Selected Products. Agritech. 2013;33(3):296–302. DOI: https://doi.org/10.22146/AGRITECH.9551.
  19. Prasetyo HA, Winardi R. Antioksidan pada Pembuatan Tepung dan Cake Ubi Jalar Ungu ( Ipomoea batatas L .). J Agrica Ekstensia. 2020;14(1):25–32.
  20. Lachman J, Hamouz K, Šulc M, Orsák M, Pivec V, Hejtmánková A, et al. Cultivar differences of total anthocyanins and anthocyanidins in red and purple-fleshed potatoes and their relation to antioxidant activity. Food Chem. 2009;114(3):836–43. DOI: https://doi.org/10.1016/J.FOODCHEM.2008.10.029.
  21. Mattioli R, Francioso A, Mosca L, Silva P. Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases. Molecules. 2020 Aug;25(17). DOI: https://doi.org/10.3390/molecules25173809.
  22. Ngestiningsih D, Rahayu RA, Batubara L. Effect of Superoxide Dismutase (SOD) Supplementation on Plasma Levels of Malondialdehyde (MDA), Total Cholesterol and LDL Cholesterol in the Elderly. J Biomed Transl Res Vol 5, No 2 December 2019. DOI: https://doi.org/10.14710/jbtr.v5i2.4679.
  23. Kim YH, Kim Y, Cho E, Kwak S, Kwon S, Bae J, et al. Alterations in intracellular and extracellular activities of antioxidant enzymes during suspension culture of sweet potato. Phytochemistry. 2004 Sep;65(17):2471–6. DOI: https://doi.org/10.1016/j.phytochem.2004.08.001.
  24. Lu X, Zhou Y, Wu T, Hao L. Ameliorative effect of black rice anthocyanin on senescent mice induced by D-galactose. Food Funct. 2014;5(11):2892–7. DOI: https://doi.org/10.1039/c4fo00391h.
  25. Zhi Q, Lei L, Li F, Zhao J, Yin R, Ming J. The anthocyanin extracts from purple-fleshed sweet potato exhibited anti-photoaging effects on ultraviolent B-irradiated BALB/c-nu mouse skin. J Funct Foods. 2020;64:103640. DOI: https://doi.org/10.1016/j.jff.2019.103640.
  26. Camkurt MA, Fındıklı E, İzci F, Kurutaş EB, Tuman TC. Evaluation of malondialdehyde, superoxide dismutase and catalase activity and their diagnostic value in drug naïve, first episode, non-smoker major depression patients and healthy controls. Psychiatry Res. 2016;238:81–5. DOI: https://doi.org/10.1016/j.psychres.2016.01.075.
  27. Kamal AA, el Khafif M, Koraah S, Massoud A, Caillard JF. Blood superoxide dismutase and plasma malondialdehyde among workers exposed to asbestos. Am J Ind Med. 1992;21(3):353–61. DOI: https://doi.org/10.1002/ajim.4700210308.
  28. Abdel-Hafez HZ, Mohamed EEM, Abd-Elghany AA. Tissue and blood superoxide dismutase activity and malondialdehyde level in leprosy. J Eur Acad Dermatology Venereol. 2010;24(6):704–8. DOI: https://doi.org/10.1111/j.1468-3083.2009.03496.x.
  29. Załuski D, Smolarz HD. Plant inhibitors of metalloproteinases and the possibility of their application in the prevention of photoaging. Ann Umcs, Pharm. 2009;22:89–96. DOI: https://doi.org/10.2478/V10080-008-0184-9.

How to Cite

Giantoro, M. ., Winaya, K. K. ., & Puspawati, N. M. D. . (2023). Purple Sweet Potato (Ipomea batatas L.) Cream Extract Increase Superoxide Dismutase (SOD) - Malondialdehyde (MDA) Ratio and Higher Collagen Expression in Rats (Rattus novergicus) Exposed to Ultraviolet B . Intisari Sains Medis, 14(3), 208–216. https://doi.org/10.15562/ism.v14i3.1860

HTML
0

Total
0

Share

Search Panel

Marvin Giantoro
Google Scholar
Pubmed
ISM Journal


Ketut Kwartantaya Winaya
Google Scholar
Pubmed
ISM Journal


Ni Made Dwi Puspawati
Google Scholar
Pubmed
ISM Journal