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Deteksi resistensi karbapenem secara fenotip dan genotip isolat Pseudomonas aeruginosa di Rumah Sakit Pendidikan Universitas Hasanuddin Makassar

  • Arthur Hugo ,
  • Yoeke Dewi Rasita ,
  • Mochammad Hatta ,
  • Baedah Madjid ,
  • Rizalinda Sjahril ,
  • Ilhamjaya Patellongi ,

Abstract

Background: Antimicrobial resistance in Pseudomonas aeruginosa is currently one of the top priorities studied by the World Health Organization (WHO). Antimicrobial resistance can be detected using both phenotyping and genotyping methods. This study presents data on Pseudomonas aeruginosa resistance to carbapenems collected from two teaching hospitals in Makassar Region, South Sulawesi, Indonesia, both phenotypically and genotypically, and also detects the OprD porin coding gene of Pseudomonas aeruginosa.

Methods: A total of 80 isolates of P. aeruginosa collected from two teaching hospitals of Hasanuddin University Makassar, which had been phenotyped for susceptibility and resistance to carbapenems (imipenem and meropenem), were retested by polymerase chain reaction (PCR) to detect the carbapenemase genes IMP, VIM, OXA-48, OXA-51 and OXA-23 carried by these bacteria.

Results: Of the 80 isolates of carbapenem-resistant Pseudomonas aeruginosa (CRPA), 24 isolates were phenotypically resistant to imipenem and meropenem. In PCR examination, 16.3% of isolates were detected to have carbapenemase coding genes IMP and 1.3% OXA-51, only 1.3% of isolates did not have the OprD porin coding gene.

Conclusion: Data on P. aeruginosa resistance both phenotypically and genotypically are expected to be a reference for consideration of clinicians in the administration of antibiotics, mostly the carbapenem group in hospitals, especially in the Makassar Region, South Sulawesi, Indonesia.

 

Latar belakang: Resistensi antimikroba pada Pseudomonas aeruginosa saat ini merupakan salah satu prioritas utama yang dikaji oleh World Health Organization (WHO). Resistensi antimikroba dapat dapat dideteksi baik menggunakan metode fenotip maupun metode genotip. Studi ini menyajikan data resistensi Pseudomonas aeruginosa terhadap golongan karbapenem yang dikumpulkan dari dua rumah sakit pendidikan di Wilayah Makassar, Sulawesi Selatan, Indonesia, baik secara fenotip maupun genotip, serta mendeteksi gen pengkode porin OprD Pseudomonas aeruginosa.

Metode: Total 80 isolat Pseudomonas aeruginosa yang dikumpulkan dari dua rumah sakit pendidikan Universitas Hasanuddin Makassar telah diketahui suseptibilitas dan resistensinya terhadap karbapenem (imipenem dan meropenem) secara fenotip, diuji kembali dengan metode polymerase chain reaction (PCR) untuk mendeteksi gen karbapenemase IMP, VIM, OXA-48, OXA-51 dan OXA-23 yang dibawa oleh bakteri ini.

Hasil: Dari 80 isolat Pseudomonas aeruginosa resisten karbapenem terdapat 24  isolat resisten terhadap imipenem dan meropenem secara fenotip. Pada pemeriksaan PCR ditemukan 16,3% isolat terdeteksi memiliki gen pengkode karbapenemase IMP dan 1,3% OXA-51, hanya 1,3% isolat yang tidak memiliki gen pengkode porin OprD.

Simpulan: Data resistensi P. aeruginosa baik secara fenotipik dan genotipik diharapkan menjadi acuan untuk menjadi pertimbangan para klinisi dalam pemberian antibiotik terutama golongan karbapenem di rumah sakit, khususnya di wilayah Makassar, Sulawesi Selatan, Indonesia.

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How to Cite

Hugo, A. ., Rasita, Y. D. ., Hatta, M. ., Baedah Madjid, Sjahril, R. ., & Patellongi, I. . (2023). Deteksi resistensi karbapenem secara fenotip dan genotip isolat Pseudomonas aeruginosa di Rumah Sakit Pendidikan Universitas Hasanuddin Makassar. Intisari Sains Medis, 14(3), 1044–1048. https://doi.org/10.15562/ism.v14i3.1856

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