Skip to main content Skip to main navigation menu Skip to site footer

Macroscopic and microscopic features of pancreatic scaffold generated by SDS-based decellularization using multiple needle injections

Abstract

Background: Pancreatic tissue engineering requires a scaffold in addition to cells and signaling. An adequate scaffold no longer contains cells but retains its extracellular matrix. Biological scaffolds from organ or tissue decellularization are widely used because they can retain the extracellular matrix essential for cell proliferation and differentiation. Pancreatic scaffolds were developed for pancreatic tissue engineering through various decellularization techniques. Available decellularization techniques have advantages and disadvantages, and the development of new techniques is necessary. This study aims to characterize pancreatic scaffold generated by SDS-based decellularization using multiple needle injections.

Method: The rat pancreas was isolated and injected with multiple SDS with graded concentration (0,1-1%) using a 1 cc syringe. Scaffold characterization was performed using hematoxylin-eosin (H&E) staining.

Results: The results obtained were the loss of cells in the pancreatic scaffold with intact extracellular matrix. Macroscopic appearance after decellularization showed a translucent, white, and flabby pancreas. Microscopic image after decellularization showed pancreatic tissue with cell loss but visible extracellular matrix.

Conclusion: This study can produce an SDS-based decellularized pancreatic scaffold using multiple needle injections with adequate macroscopic and microscopic characteristics.

References

  1. Asril NM, Tabuchi K, Tsunematsu M, Kobayashi T, Kakehashi M. Predicting healthy lifestyle behaviors among patients with type 2 diabetes in Rural Bali, Indonesia. Clin Med Insights Endocrinol Diabetes. 2020; 13: 1–13.
  2. Gotera W, Wulandari DC. Metabolic disorders among tourism hotel workers in Nusa Dua, Bali. Biomedical and Pharmacology Journal. 2017; 10(4): 1805–1808.
  3. Peloso A, Citro A, Zoro T, Cobianchi L, Kahler-Quesada A, Bianchi CM, et al. Regenerative medicine and diabetes: Targeting the extracellular matrix beyond the stem cell approach and encapsulation technology. Frontiers in Endocrinology. 2018; 9(445): 1–9.
  4. Costa A, Naranjo JD, Londono R, Badylak SF. Biologic scaffolds. Cold Spring Harb Perspect Med. 2017; 7(9): 1–23.
  5. Antarianto RD, Dewi AAAAP, Pragiwaksana A, Pawitan JA. Decellularization of liver cubes using multiple site syringe injection for generating native liver scaffold: Preliminary report. In: AIP Conference Proceedings. American Institute of Physics Inc., 2019, pp. 1–6.
  6. Wardhana A, Valeria M. Tissue engineering and regenerative medicine: A Review. Jurnal Plastik Rekonstruksi. 2020; 7(1): 10–17.
  7. Ren H, Shi X, Tao L, Xiao J, Han B, Zhang Y, et al. Evaluation of two decellularization methods in the development of a whole-organ decellularized rat liver scaffold. Liver Internasional. 2013; 33(3): 448–458.
  8. Zhou P, Huang Y, Guo Y, Wang L, Ling C, Guo Q, et al. Decellularization and Recellularization of Rat Livers With Hepatocytes and Endothelial Progenitor Cells. Artif Organs. 2016; 40(3): 25–38.
  9. Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006; 27(19): 3675–3683.
  10. Gazia C, Gaffley M, Asthana A, Chaimov D, Orlando G. Scaffolds for pancreatic tissue engineering. Handbook of Tissue Engineering Scaffolds. 2019; 2: 765–786.
  11. Shamis Y, Hasson E, Soroker A, Bassat E, Shimoni Y, Ziv T, et al. Organ-specific scaffolds for in vitro expansion, differentiation, and organization of primary lung cells. Tissue Eng Part C Methods. 2011; 17(8): 861–870.
  12. Cortiella J, Niles J, Cantu A, Brettler A, Pham A, Vargas G, et al. Influence of acellular natural lung matrix on murine embryonic stem cell differentiation and tissue formation. Tissue Eng Part A. 2010; 16(8): 2565–2580.
  13. Yu H, Chen Y, Kong H, He Q, Sun H, Bhugul PA, et al. The rat pancreatic body tail is a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering. J Biol Eng. 2018; 12(1): 1–15.
  14. Llacua LA, Faas MM, de Vos P. Extracellular matrix molecules and their potential contribution to the function of transplanted pancreatic islets. Diabetologia. 2018; 61(6): 1261–1272.
  15. Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. 2011; 32(12): 3233–3243.
  16. Mirmalek-Sani SH, Orlando G, McQuilling JP, Pareta R, Mack DL, Salvatori M, et al. Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. Biomaterials. 2013; 34(22): 5488–5495.
  17. Salvatori M, Katari R, Patel T, Peloso A, Mugweru J, Owusu, K, et al. Extracellular matrix scaffold technology for bioartificial pancreas engineering: State of the art and future challenges. Journal of Diabetes Science and Technology. 2014; 8(1): 159–169.
  18. Townsend SE, Gannon M. Extracellular Matrix-Associated Factors Play Critical Roles in Regulating Pancreatic β-Cell Proliferation and Survival. Endocrinology. 2019; 160(8): 1885–1894.
  19. Klak M, Łojszczyk I, Berman A, Tymicki G, Adamiok-Ostrowska A, Sierakowski M, et al. Impact of porcine pancreas decellularization conditions on the quality of obtained decm. Int J Mol Sci. 2021; 22(13): 1–16.
  20. Sackett SD, Tremmel DM, Ma F, Feeney AK, Maguire RM, Brown ME, et al. Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas. Sci Rep. 2018; 8(1): 1–16.

How to Cite

AA Ayu Asri Prima Dewi, Komang Trisna Sumadewi, Putu Diah Witari, Fransiscus Fiano Anthony Kerans, Luh Gde Evayanti, & Dewa Ayu Agung Alit Suka Astini. (2023). Macroscopic and microscopic features of pancreatic scaffold generated by SDS-based decellularization using multiple needle injections. Intisari Sains Medis, 14(3), 1028–1031. https://doi.org/10.15562/ism.v14i3.1843

HTML
0

Total
0

Share

Search Panel

AA Ayu Asri Prima Dewi
Google Scholar
Pubmed
ISM Journal


Komang Trisna Sumadewi
Google Scholar
Pubmed
ISM Journal


Putu Diah Witari
Google Scholar
Pubmed
ISM Journal


Fransiscus Fiano Anthony Kerans
Google Scholar
Pubmed
ISM Journal


Luh Gde Evayanti
Google Scholar
Pubmed
ISM Journal


Dewa Ayu Agung Alit Suka Astini
Google Scholar
Pubmed
ISM Journal