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Uji daya hambat minyak atsiri kulit buah jeruk bali (Citrus maxima) terhadap bakteri Pseudomonas aeruginosa



Abstract

Background: Pseudomonas aeruginosa is a gram-negative bacterium that is commonly associated with Healthcare-Associated infections (HAI), particularly Multidrug-resistance (MDR) P. aeruginosa. The polyphenolic compounds found in the peel of pomelo (C. maxima) are reported to have shown an effective antimicrobial effect. Therefore, this study aimed to investigate the inhibitory effect of C. maxima peel essential oil on the growth of P. aeruginosa bacteria.

Methods: This in vitro study evaluates the inhibitory effect of C. maxima peel essential oil against clinical isolate strains (SP47) and MDR P. aeruginosa by measuring the diameter of the inhibition zone (mm). Five series of essential oil concentrations (20%, 40%, 60%, 80%, and 100%) and the control group were used in the paper disk diffusion test with three repetitions (triplet). All data were statistically analyzed using SPSS ver. 20.

Results: A total of 4.85 mL of essential oil from 7.2 kg of C. maxima fruit peel was obtained. Inhibition zones were observed in clinical isolates of P. aeruginosa by concentrations of 60%, 80%, 100%, and K+ with means of 6.27±0.08 mm; 6.71±0.19 mm; 9.08±0.29 mm; and 40.8±0.48 mm, respectively. In MDR P. aeruginosa, the inhibition zone by concentrations of 80%, 100%, and K+ with means of 6.45±0.13 mm; 7.15±0.13 mm; and 9.08±0.34 mm, respectively. There was a significant difference in the mean diameter within groups (p<0.05).

Conclusion: C. maxima fruit peel essential oil was able to inhibit the growth of P. aeruginosa bacteria, both clinical and MDR isolates. There were differences in inhibition between the concentration series.

 

Metode: Penelitian ini merupakan studi in vitro yang mengevaluasi daya hambat minyak atsiri kulit buah C. maxima terhadap strain isolat klinis (SP47) dan MDR P. aeruginosa melalui pengukuran diameter zona hambat (mm). Sebanyak lima seri konsentrasi minyak atsiri (20%, 40%, 60%, 80%, dan 100%) dan kelompok kontrol digunakan dalam uji paper disk diffusion dengan pengulangan sebanyak tiga kali (triplet) Seluruh data dianalisis secara statistik menggunakan SPSS ver. 20.

Hasil: Sebanyak 4,85 mL minyak atsiri dari 7,2 kg kulit buah C. maxima diperoleh dengan metode destilasi uap. Zona hambat teramati pada isolat klinis P. aeruginosa oleh konsetrasi 60%, 80%, 100%, dan K+ dengan rerata sebesar 6,27±0,08 mm; 6,71±0,19 mm; 9,08±0,29 mm; dan 40,8±0,48 mm berturut-turut. Zona hambat juga teramati pada isolat MDR P. aeruginosa oleh konsetrasi 80%, 100%, dan K+ dengan rerata sebesar 6,45±0,13 mm; 7,15±0,13 mm; dan 9,08±0,34 mm berturut-turut. Terdapat perbedaan rerata diameter yang signifikan antar kelompok uji (p<0,05).

Simpulan: Minyak atsiri kulit buah C. maxima mampu menghambat pertumbuhan bakteri P. aeruginosa baik isolat klinis maupun MDR. Adapun terdapat perbedaan daya hambat antar seri konsentrasi.

Kata kunci: antimikroba, citrus maxima, minyak atsiri, multidrug resistant

Keywords: antimikroba citrus maxima minyak atsiri multidrug resistant

References

  1. Khan HA, Baig FK, Mehboob R. Nosocomial infections: Epidemiology, prevention, control and surveillance. Asian Pac J Trop Biomed. 2017;7(5):478–82 http://dx.doi.org/10.1016/j.apjtb.2017.01.019
  2. CDC. National and State Associated Infections Progress Report. Centers Dis Control Prev. 2016;(March).
  3. Duerink DO, Roeshadi D, Wahjono H, Lestari ES, Hadi U, Wille JC, et al. Surveillance of healthcare-associated infections in Indonesian hospitals. J Hosp Infect. 2015;62(2):219–29 https://doi.org/10.1016/j.jhin.2005.08.004
  4. Estiningsih D, Puspitasari I, Nuryastuti T. Identifikasi Infeksi Multidrug-Resistant Organisms pada Pasien yang dirawat di Bangsal Neonatal Intensive Care Unit (NICU) RSUP DR. Soeradji Tirtonegoro Klaten. J Manaj dan Pelayanan Farm. 2016;6(3):243–8 https://doi.org/10.22146/jmpf.351
  5. Ciofu O, Tolker-Nielsen T. Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents—How P. aeruginosa Can Escape Antibiotics [Internet]. Vol. 10, Frontiers in Microbiology . 2019. p. 913 https://www.frontiersin.org/article/10.3389/fmicb.2019.00913
  6. Kostylev M, Kim DY, Smalley NE, Salukhe I, Greenberg EP, Dandekar AA. Evolution of the Pseudomonas aeruginosa quorum-sensing hierarchy. Proc Natl Acad Sci U S A. 2019;116(14):7027–32 https://doi.org/10.1073/pnas.1819796116
  7. Thi MTT, Wibowo D, Rehm BHA. Pseudomonas aeruginosa Biofilms. Int J Mol Sci. 2020;21(22) https://doi.org/10.3390/ijms21228671
  8. Bheemavarapu H, Arief M, Ahmad A, Patel I. Nosocomial Infections in India: The Unaddressed Lacunae! J Pharm Pract Community Med. 2018;4(2):43–43 https://doi.org/10.5530/jppcm.2018.2.11
  9. Guzzo F, Scognamiglio M, Fiorentino A, Buommino E, D’Abrosca B. Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms. Molecules. 2020;25(21) https://doi.org/10.3390/molecules25215024
  10. Sahlan M, Damayanti V, Tristantini D, Hermansyah H, Wijanarko A, Olivia Y. Antimicrobial activities of pomelo (Citrus maxima) seed and pulp ethanolic extract. AIP Conf Proc. 2018;1933(February):10–6 https://doi.org/10.1063/1.5023949
  11. Br Karo RM, Manalu P, Sinurat JP. Antibacterial Activity of Flavonoid-Rich Fractions Of Citrus Maxima Peel Extract. Stannum J Sains dan Terap Kim. 2020;2(2):16–21 https://doi.org/10.33019/jstk.v2i2.1977
  12. Lan-Phi NT, Vy TT. Chemical composition, antioxidant and antibacterial activities of peels’ essential oils of different pomelo varieties in the south of Vietnam. Int Food Res J. 2015;22(6):2426–31 ISSN: 1985-4668
  13. Suwandi. Outlook Komoditas Pertanian Subsektor Hortikultura Jeruk 2015. Badan Pus Stat. 2015;1–88.
  14. Ma’rufah T, Hertiani T, Anshory H. Perbandingan Daya Antiquorum Sensing Ekstrak N-Heksan, Etil Asetat Dan Metanol Kulit Batang Krangean (Litsea cubeba (Lour.) Pers.) Terhadap Pseudomonas aeruginosa. J Ilm Farm. 2013;10(1):7–17.
  15. Das S, Borah M, Ahmed S. Antibacterial activity of the ethanolic extract of leaves of Citrus maxima (Burm.) Merr. on escherichia coli and pseudomonas aeruginosa. Asian J Pharm Clin Res. 2013;6(SUPPL.4):136–9.
  16. Singh A. Evaluation of Antimicrobial Potential and Phytochemical Assessment of Citrus maxima Burm . Seeds Extracts Against Respiratory Tract Pathogens. 2016;(October). https://10.7537/marsnys090916.02.
  17. Abirami A, Nagarani G, Siddhuraju P. In vitro antioxidant, anti-diabetic, cholinesterase and tyrosinase inhibitory potential of fresh juice from Citrus hystrix and C. maxima fruits. Food Sci Hum Wellness. 2014;3(1):16–25 https://doi.org/10.1016/j.fshw.2014.02.001
  18. Abdelhady ASM, Darwish NM, Abdel-rahman SM, Abo NM, Magd E. The combined antimicrobial activity of citrus honey and fosfomycin on multidrug resistant Pseudomonas aeruginosa isolates. 2020;6(2):162–75 https://doi.org/10.3934/microbiol.2020011
  19. Ayad AC, Buerano KN, Andrew S, Sormin S, Malabat C, Jael S, et al. Antibacterial efficiency of pomelo peel extract on various concentrations against selected microorganisms. 2015; https://doi.org/10.35974/ISC.V5I1.1560
  20. Rammohan A, Reddy GM, Bhaskar BV, Gunasekar D, Zyryanov G V. Phytochemistry and pharmacological activities of the genus Rhynchosia: a comprehensive review. Planta. 2019;251(1):9 https://doi.org/10.1007/s00425-019-03311-2
  21. Ghomi JS, Masoomi R, Kashi FJ, Batooli H. In vitro bioactivity of essential oils and methanol extracts of Salvia reuterana from Iran. Nat Prod Commun. 2012;7(5):651–4 https://doi.org/10.1177/1934578X1200700527
  22. Khan NH, Qian CJ, Perveen N. Phytochemical screening , antimicrobial and antioxidant activity determination of citrus maxima peel. IEEE Eng Med Biol Mag. 2019;6(4):279–85 https://doi.org/10.15406/ppij.2018.06.00187
  23. Tsai M-L, Lin C-D, Khoo KA, Wang M-Y, Kuan T-K, Lin W-C, et al. Composition and Bioactivity of Essential Oil from Citrus grandis (L.) Osbeck “Mato Peiyu” Leaf. Molecules. 2017;22(12) https://doi.org/10.3390/molecules22122154
  24. Huỳnh XP, Lưu MC, Trần TXN, Nguyễn NT, Bùi HĐL, Bạch LG, et al. Chemical composition and antimicrobial activity of essential oil extracted from pomelo (Citrus maxima (Burm.) Merr.). Hue Univ J Sci Nat Sci. 2021;130(1C SE-):75–83 https://doi.org/10.26459/hueunijns.v130i1C.6247
  25. Karakaya H, Ozturk FS, Koc TB, Yasar K. Chemical Composition and Antimicrobial Activity of Pummelo (Citrus maxima) Essential Oil Derived from Fruit Peel. J Essent Oil Bear Plants. 2022;25(3):524–35 https://doi.org/10.1080/0972060X.2022.2100229

How to Cite

Sadeva, I. G. K. A., Budayanti, N. N. S., Hendrayana, M. A., & Sukrama, I. D. M. (2023). Uji daya hambat minyak atsiri kulit buah jeruk bali (Citrus maxima) terhadap bakteri Pseudomonas aeruginosa. Intisari Sains Medis, 14(1), 124–130. https://doi.org/10.15562/ism.v14i1.1557
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I Gede Krisna Arim Sadeva
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Ni Nyoman Sri Budayanti
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Made Agus Hendrayana
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I Dewa Made Sukrama
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