Review Article

Microstructural aspect of pineal body: pinealocytes population

Gede Wirata , Putu Nia Calista Santoso, Putu Ayu Utami Dewantari

Gede Wirata
Anatomy Department, Faculty of Medicine, Udayana University. Email:

Putu Nia Calista Santoso
Bachelor of Science Degree in Biology, Francisco J. Ayala School of Biological Sciences, University of California Irvine

Putu Ayu Utami Dewantari
Udayana University Hospital
Online First: January 25, 2018 | Cite this Article
Wirata, G., Santoso, P., Dewantari, P. 2018. Microstructural aspect of pineal body: pinealocytes population. Intisari Sains Medis 9(1). DOI:10.15562/ism.v9i1.151

Background: Pineal tissue was compound with 2 types of cell known as pinealocytes and astrocyte cell. Furthermore, microanatomical research of pineal body is a rare procedure conducted for several years even in Indonesia, and its anatomical aspect needs to be explained.  Aim: enrich the scope of knowledge related to mammalian pineal within the pineal body. Methods: 3 Wistar rats (Rattus novergicus), especially their pineal tissue, was extracted by pinealectomy method as a histotechnical procedure in a laboratory setting was prepared. Result: Pinealocytes characteristic are basophilic, large, regularly nucleus and a lot of mitochondria. On the other side, astrocyte has longer cytoplasmic processus found around the perivascular area and among the pinealocytes one. Conclusion: The quantities of pinealocytes appear as a most dominant cell that fills the inner part of the pineal body and probable to synchronize the production of melatonin and entirely supported by their neighbor, astrocyte cells.


Keywords: histotechnical method, pinealectomy, epiphysis



Sahai, Ashok. 2011. Pineal Gland History & Lesser Known Facts [powerpoint in PDF]. India: King George’s Medical Faculty.

Axelrod J. The Pineal Gland: A Neurochemical Transducer [Artikel]. Science 1974; 184 (Issue 4144): 1341-8

Standering S. “Ventricular System & Subarachnoid Space”, chapter 10, pp. 240-241, “Diencephalon”, chapter 21, p. 324, “The Nervous System” chapter 24, p. 380, “Eye”, chapter 40, p. 692. In: Gray’s Anatomy: The Anatomical Basis of Clinical Practice. 40th ed. Churchill Livingstone: Elsevier; 2008.

Al Husain SM. The Pinealocytes of the Human Pineal Gland: A Light and Electron Microscopic Study. Folia Morph 2006; 65 (3): 181-7

Wurtman RJ, Julius Axelrod. 1965. The Pineal Gland [Article]. [Accesed on: 18 February 2017] URL : [ ]

Srinivasan V. The Pineal Gland: Its Physiological and Pharmacological Role [Review Article]. Ind. J. Physiol. Pharmac 1989; 33 (No. 4): 263-72

Manca ME, Manunta ML, Spezzigu A, Torres-Rovira L, Gonzales-Bulnes A, Pasciu V, Melatonin Deprivation Modifies Follicular and Corpus Luteal Growth Dynamics in a Sheep Model. Reproduction 2014; 147: 885-95

Olcese J. Melatonin Receptors in The Human Reproductive Tract [Article] 2007; 47-56.

Cutando A, Lopez-Valverde A, Arias-Santiago S, Vicente JD, De Diego RG. Role of Melatonin in Cancer Treatment [Review]. Anticancer Research 2012; 32: 2747-54

Renzi A, Glasser S, DeMorrow S, Mancinelli R, Meng F, Franchitto A, Melatonin Inhibits Cholangiocyte Hyperplasia in Cholestatic Rats by Interaction with MT1 but not MT2 Melatonin Receptors. Ann J Physiol Gastrointest Liver Physiol 2011; 301: 634-43

Han Y, DeMorrow S, Invernizzi P, Jing Qi, Glaser S, Renzi A, Melatonin Exerts by an Autocrine Loop Antiproliferative Effects in Cholangiocarcinoma; Its Synthesis is Reduced Favoring Cholangiocarcinoma Growth. Ann J Physiol Gastrointest Liver Physiol 2011; 301: 623-33

Zats M, Gastel JA, Heath III JR, Klein DC. Chick Pineal Melatonin Synthesis: Light and Cyclic AMP Control Abundance of Serotonin N-Acetyltransferase Protein. J. Neurochem 2000; 74: 2315-21

Zhang B-L, Zannou E, Sannajust F. Effects of Photoperiod Reduction on Rat Circadian Rhythms of BP, Heart Rate, and Locomotor Activity. Am J Physiol Regulatory Integrative Comp Physiol 2000; 279: 169-78

Cook JS, Chester AR. Melatonin Attenuates The Vestibulosympathetic but not Vestibulocollic Reflexes in Humans: Selective Impairment of the Utricles. J Appl Physiol 2010; 109: 1697 – 1701

Purucker G. Man in Evolution. Chapter 16. The Pineal and Pituitary Glands. Theosophical University Press; 2011: 208.

Lokhorst GJ. Descartes and the Pineal Gland. USA: Stanford Encyclopedia of Philosophy; 2013. [Accesed on 7 March 2017] URL:

Vollrath L. Comparative Morphology of the Vertebrate Pineal Complex. Dalam Kappers JA, Pevet P, editor. The Pineal Gland of Vertebrates Including Man [e-book]. Elsevier: Progress in Brain Research; 2011: Vol 53: 25-38

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