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Hubungan varikokel dengan indeks fragmentasi DNA spermatozoa pada laki-laki infertil di RSIA Puri Bunda, Denpasar, Indonesia

Abstract

Background: Varicoceles can affect the process of spermatogenesis, causing changes in semen parameters and the DNA integrity of spermatozoa. The examination method was optimized by examining the DNA fragmentation of spermatozoa. Spermatozoa DNA fragmentation examination can be performed on men with risk factors for advanced age, obesity, smoking, and exposure to gonadotoxins and varicocele. This study aims to determine the relationship between varicocele and sperm DNA fragmentation index in infertile men at RSIA Puri Bunda Denpasar.

Methods: This research is an analytic study with a cross sectional method. Sampling in this study used patient medical records at RSIA Puri Bunda Denpasar with a purposive sampling method based on inclusion and exclusion criteria in the population, obtaining as many as 50 patient data. Data were analyzed using SPSS version 25 for Windows.

Results: From 50 samples, it was found that severe DFI results were more common in samples of patients with varicocele, as many as 24 samples (80.0%) and 11 samples (55.0%) in men without varicocele. Mild DFI was dominated by men without varicocele, as many as 9 samples (45.0%) and 6 samples (20.0%) in men with varicocele. The statistical test results using the Yates test (Continuity correction) obtained a p-value of 0.115, which indicates p> 0.05.

Conclusion: There is no significant relationship between varicocele and spermatozoa DNA fragmentation index in infertile men at RSIA Puri Bunda Denpasar.

 

Latar Belakang: Varikokel dapat memengaruhi proses spermatogenesis sehingga meyebabkan perubahan parameter semen dan integritas DNA spermatozoa. Metode pemeriksaan dioptimalkan dengan melakukan pemeriksaan fragmentasi DNA spermatozoa. Pemeriksaan fragmentasi DNA spermatozoa dapat dilakukan pada laki-laki yang memiliki faktor risiko usia lanjut, obesitas, merokok, paparan gonadotoxin dan varikokel. Penelitian ini bertujuan untuk mengetahui hubungan antara varikokel dengan indeks fragmentasi DNA spermatozoa pada laki-laki infertil di RSIA Puri Bunda Denpasar.

Metode: Penelitian ini merupakan penelitian analitik dengan metode potong lintang. Pengambilan sampel pada penelitian ini menggunakan rekam medis pasien di RSIA Puri Bunda Denpasar dengan metode purposive sampling berdasarkan kriteria inklusi dan eksklusi pada populasi. Keseluruhan diperoleh sebanyak 50 data pasien. Data dianalisis dengan SPSS versi 25 untuk Windows.

Hasil: Dari 50 sampel diketahui bahwa hasil DFI berat lebih banyak terjadi pada sampel penderita varikokel, yaitu sebanyak 24 sampel (80,0%) dan sebanyak 11 sampel (55,0%) terjadi pada laki-laki tidak varikokel. DFI ringan didominasi oleh laki-laki tidak varikokel sebanyak 9 sampel (45,0%) dan 6 sampel (20,0%) pada laki-laki varikokel. Hasil uji statistik dengan menggunakan uji Yates (Continuity correction) diperoleh nilai p sebesar 0,115 yang menandakan p>0,05.

Simpulan: Tidak terdapat hubungan yang bermakna antara varikokel dengan indeks fragmentasi DNA spermatozoa pada laki-laki infertil di RSIA Puri Bunda Denpasar.

References

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How to Cite

Pretamiswari, A. A. A., Pramesemara, I. G. N., Kurniawan, Y., & Ratnayanti, I. G. A. D. (2022). Hubungan varikokel dengan indeks fragmentasi DNA spermatozoa pada laki-laki infertil di RSIA Puri Bunda, Denpasar, Indonesia. Intisari Sains Medis, 13(2), 649–653. https://doi.org/10.15562/ism.v13i2.1360

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Anak Agung Ayu Pretamiswari
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I Gusti Ngurah Pramesemara
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Yukhi Kurniawan
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I Gusti Ayu Dewi Ratnayanti
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