Skip to main content Skip to main navigation menu Skip to site footer

Kadar heat shock protein 70 (HSP 70) yang tinggi pada serum ibu sebagai faktor risiko persalinan preterm

  • Tjokorda G. A. Suwardewa ,
  • Ketut Surya Negara ,
  • Anak Agung Ngurah Anantasika ,
  • I Wayan Artana Putra ,
  • I Gde Sastra Winata ,
  • Piere Emanuel Yoltuwu ,

Abstract

Introduction: Preterm birth defined as parturition that occurs less than 37 completed weeks of gestation is still being a big problem in obstetrics, especially in perinatology. Preterm delivery is one of the most common causes of neonatal morbidity and mortality. This study examined serum HSP 70 levels as a risk factor for preterm delivery.

Method: The research design was analytic with a case-control method at Sanglah Hospital from February to June 2021. The samples were divided into two groups which are in the case group was 30 samples and the control group was 30 samples. In both groups, HSP 70 levels were collected by taking 5cc of blood sample from cubital vein. Furthermore, the examination was carried out using the enzyme-link immunosorbent assay (ELISA) method at Biomedik Terpadu Laboratory service.

Result: Based on the data on the characteristics of the subjects, respectively maternal age, gestational age, BMI, and parity there was no significant differences between two group. Serum HSP levels were significantly different between the two groups with OR 4.030 (95%CI: 1,372-11,84; p-value 0.01). The cut-off value for serum HSP 70 levels was 12.85 ng/ml, with a sensitivity of 70%, specificity of 63.3%, and an area under the curve (AUC) value of 0.807 (95% CI 0.697 – 0.916, p-value <0.001).

Conclusion: This study revealed that high levels of HSP 70 in serum is a risk factor of preterm labor. High blood serum levels of HSP 70 could be a reference in determining high risk of preterm labor in pregnant women.

 

 

Pendahuluan: Persalinan preterm didefinisikan kelahiran sebelum usia gestasi kurang dari 37 minggu masih menjadi masalah besar dalam bidang obstetri khususnya dibidang perinatologi. Persalinan preterm merupakan salah satu penyebab utama tingginya angka morbiditas dan mortalitas neonatus. Penelitian ini meneliti mengenai kadar HSP 70 pada serum ibu sebagai faktor risiko persalinan preterm.

Metode: Rancangan penelitian yang digunakan adalah analitik dengan metode kasus-kontrol, dilakukan di RSUP Sanglah pada periode Februari 2021 sampai Juni 2021. Sampel dibagi menjadi 2 kelompok yakni kelompok kasus sebanyak 30 sampel dan kontrol 30 sampel. Pada kedua kelompok, pemeriksaan kadar HSP 70 dilakukan melalui pengambilan sampel darah 5 ml pada vena cubiti. Selanjutnya dilakukan pemeriksaan dengan metode enzyme-link immunosorbent assay (ELISA) di Unit Laboratorium Biomedik Terpadu.

Hasil: Data karakteristik subyek yaitu usia ibu, usia kehamilan, IMT dan jumlah paritas, tidak ditemukan adanya perbedaan bermakna. Kadar serum HSP yang tinggi berbeda bermakna antara kedua kelompok dengan nilai OR 4.030 (IK 95%: 1,372-11,84; nilai p 0.01). Nilai cut off kadar serum HSP 70 sebesar 12.85 ng/ml, dengan sensitifitas sebesar 70%, spesifisitas 63,3%, dan nilai area under the curve (AUC) sebesar 0,807 (IK 95% 0,697 – 0,916, nilai p <0,001).

Simpulan: Penelitian ini menunjukkan bahwa kadar HSP 70 yang tinggi pada serum ibu sebagai faktor risiko kejadian terjadinya persalinan preterm. Nilai kadar HSP 70 yang tinggi dapat menjadi suatu acuan dalam menentukan risiko tinggi kejadian persalinan preterm pada ibu hamil.

Section

References

  1. Adams Waldorf KM, Singh N, Mohan AR, Young RC, Ngo L, Das A, et al. Uterine overdistention induces preterm labor mediated by inflammation: observations in pregnant women and nonhuman primates. Am J Obstet Gynecol. 2015/08/15. 2015;213(6):830.e1-830.e19. Available from: https://pubmed.ncbi.nlm.nih.gov/26284599
  2. Blencowe H, Cousens S, Chou D, Oestergaard M, Say L, Moller A-B, et al. Born too soon: the global epidemiology of 15 million preterm births. Reprod Health. 2013/11/15. 2013;10 Suppl 1(Suppl 1):S2–S2. Available from: https://pubmed.ncbi.nlm.nih.gov/24625129
  3. Molvarec A, Tamási L, Losonczy G, Madách K, Prohászka Z, Rigó Jr J. Circulating heat shock protein 70 (HSPA1A) in normal and pathological pregnancies. Cell Stress Chaperones. 2009/10/12. 2010;15(3):237–47. Available from: https://pubmed.ncbi.nlm.nih.gov/19821.
  4. Ferat-Osorio E, Sánchez-Anaya A, Gutiérrez-Mendoza M, Boscó-Gárate I, Wong-Baeza I, Pastelin-Palacios R, et al. Heat shock protein 70 down-regulates the production of toll-like receptor-induced pro-inflammatory cytokines by a heat shock factor-1/constitutive heat shock element-binding factor-dependent mechanism. J Inflamm (Lond). 2014;11:19. Available from: https://pubmed.ncbi.nlm.nih.gov/25053922
  5. Mambula SS, Calderwood SK. Heat Shock Protein 70 Is Secreted from Tumor Cells by a Nonclassical Pathway Involving Lysosomal Endosomes. J Immunol. 2006;177(11):7849–57. Available from: http://dx.doi.org/10.4049/jimmunol.177.11.7849
  6. Hromadnikova I, Dvorakova L, Kotlabova K, Kestlerova A, Hympanova L, Novotna V, et al. Circulating heat shock protein mRNA profile in gestational hypertension, pre-eclampsia & foetal growth restriction. Indian J Med Res. 2016;144(2):229–37. Available from: https://pubmed.ncbi.nlm.nih.gov/27934802
  7. Kasan Wiharjo S, Hari Respati S, Budi Giri Bawono A. Kadar Heat Shock Protein 70 pada Persalinan Prematur. J Kedokt Brawijaya. 2015;28(4):309–13. Available from: http://dx.doi.org/10.21776/ub.jkb.2015.028.04.9
  8. Molvarec A, Prohászka Z, Nagy B, Szalay J, Füst G, Karádi I, Rigó J Jr. Association of elevated serum heat-shock protein 70 concentration with transient hypertension of pregnancy, preeclampsia and superimposed preeclampsia: a case-control study. J Hum Hypertens. 2006;20(10):780-6. doi: 10.1038/sj.jhh.1002060.
  9. Chang A, Zhang Z, Jia L, Zhang L, Gao Y, Zhang L. Alteration of heat shock protein 70 expression levels in term and preterm delivery. J Matern Neonatal Med. 2013;26(16):1581–5. Available from: http://dx.doi.org/10.3109/14767058.2013.795535
  10. Fukushima A, Kawahara H, Isurugi C, Syoji T, Oyama R, Sugiyama T, et al. Changes in serum levels of heat shock protein 70 in preterm delivery and pre-eclampsia. J Obstet Gynaecol Res. 2005;31(1):72–7. Available from: http://dx.doi.org/10.1111/j.1447-0756.2005.00244.x
  11. Chaiworapongsa T, Erez O, Kusanovic JP, Vaisbuch E, Mazaki-Tovi S, Gotsch F, et al. Amniotic fluid heat shock protein 70 concentration in histologic chorioamnionitis, term and preterm parturition. J Matern Fetal Neonatal Med. 2008;21(7):449–61. Available from: https://pubmed.ncbi.nlm.nih.gov/18570125
  12. Molvarec A, Rigó J, Nagy B, Walentin S, Szalay J, Füst G, et al. Serum heat shock protein 70 levels are decreased in normal human pregnancy. J Reprod Immunol. 2007;74(1–2):163–9. Available from: http://dx.doi.org/10.1016/j.jri.2006.12.002
  13. Jean-Pierre C, Perni SC, Bongiovanni AM, Kalish RB, Karasahan E, Ravich M, et al. Extracellular 70-kd heat shock protein in mid-trimester amniotic fluid and its effect on cytokine production by ex vivo–cultured amniotic fluid cells. Am J Obstet Gynecol. 2006;194(3):694–8. Available from: http://dx.doi.org/10.1016/j.ajog.2006.01.066

How to Cite

Suwardewa, T. G. A., Negara, K. S., Anantasika, A. A. N., Artana Putra, I. W., Winata, I. G. S., & Yoltuwu, P. E. (2022). Kadar heat shock protein 70 (HSP 70) yang tinggi pada serum ibu sebagai faktor risiko persalinan preterm. Intisari Sains Medis, 13(1), 181–184. https://doi.org/10.15562/ism.v13i1.1329

HTML
38

Total
31

Share

Search Panel

Tjokorda G. A. Suwardewa
Google Scholar
Pubmed
ISM Journal


Ketut Surya Negara
Google Scholar
Pubmed
ISM Journal


Anak Agung Ngurah Anantasika
Google Scholar
Pubmed
ISM Journal


I Wayan Artana Putra
Google Scholar
Pubmed
ISM Journal


I Gde Sastra Winata
Google Scholar
Pubmed
ISM Journal


Piere Emanuel Yoltuwu
Google Scholar
Pubmed
ISM Journal