Research Article

Perbandingan kadar Thyroid Stimulating Hormone (TSH) dan kadar Free T4 (FT4) antara metode Fluorescence Immunoassay (FIA) dan metode Electrochemiluminescence Immunoassay (ECLIA) di RSUP Sanglah, Bali, Indonesia

Ekarini Katharina Yunarti Nabu , Sianny Herawati, Ni Kadek Mulyantari, Anak Agung Wiradewi Lestari, I Putu Yuda Prabawa

Ekarini Katharina Yunarti Nabu
Program Studi Pendidikan Dokter Spesialis Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia. Email: katharinanewar15@gmail.com

Sianny Herawati
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia

Ni Kadek Mulyantari
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia

Anak Agung Wiradewi Lestari
Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia

I Putu Yuda Prabawa
Program Studi Pendidikan Dokter Spesialis Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia Departemen Patologi Klinik, Fakultas Kedokteran, Universitas Udayana, RSUP Sanglah, Bali, Indonesia
Online First: August 31, 2021 | Cite this Article
Nabu, E., Herawati, S., Mulyantari, N., Lestari, A., Prabawa, I. 2021. Perbandingan kadar Thyroid Stimulating Hormone (TSH) dan kadar Free T4 (FT4) antara metode Fluorescence Immunoassay (FIA) dan metode Electrochemiluminescence Immunoassay (ECLIA) di RSUP Sanglah, Bali, Indonesia. Intisari Sains Medis 12(2): 613-616. DOI:10.15562/ism.v12i2.1062


Background: Diagnostic and therapeutic cases of hyperthyroidism can be identified by examining the levels of Thyroid Stimulating Hormone (TSH) and Free T4 (FT4). There are several examination methods, namely the Fluorescence Immunoassay (FIA) and the Electro-Chemiluminescence Immunoassay (ECLIA) method. This study aims to evaluate the difference in TSH and FT4 level in the FIA method and ECLIA method.

Method: This study used observational analytic methods cross sectional design, with 45 samples serum patient with hyperthyroid in January-March 2019. Examine level TSH and FT4 levels with FIA methods (NANO ENTEK FRENDTM) and ECLIA method (Cobas e601 ROCHE). Data were analyzed using SPSS version 17 for Windows.

Results: Most of the respondents were aged 25-34 years (28.8%) and female (86.7%). There was no significant difference in the mean TSH level on examination using the ECLIA method (0.60±0.79 mIU/L) and the FIA method (0.63±0.76 mIU/L) (p=0.639). In addition, there was no significant difference in FT4 both in the examination using the ECLIA method (1.67±1.05 ng/dl) and the FIA method (1.69±1.72 ng/dl) (p=0.745).

Conclusions: There was no significant difference between TSH and FT4 in both ECLIA and FIA methods.

 

Latar Belakang: Diagnosis maupun pemantauan terapi kasus hipertiroid dapat diketahui dengan pemeriksaan kadar hormon Thyroid Stimulating Hormone (TSH) dan Free T4 (FT4). Terdapat beberapa metode pemeriksaan yaitu metode Fluorescence Immunoassay (FIA) dan metode Electro-Chemiluminescence Immunoassay (ECLIA). Penelitian ini bertujuan untuk mengevaluasi perbedaan kadar TSH dan FT4 metode FIA dan metode ECLIA.

Metode: Penelitian ini menggunakan metode observasional analitik dengan desain potong lintang pada 45 sampel serum penderita hipertiroid selama periode Januari-Maret 2019. Dilakukan pemeriksaan kadar TSH dan FT4 dengan metode FIA (NANO ENTEK FRENDTM) dan metode ECLIA (Cobas e601 Roche). Data dianalisis dengan SPSS versi 17 untuk Windows.

Hasil: Sebagian besar responden berusia 25-34 tahun (28,8%) dan berjenis kelamin perempuan (86,7%). Tidak terdapat perbedaan bermakna rerata kadar TSH pada pemeriksaan menggunakan metode ECLIA (0,60±0,79 mIU/L) maupun metode FIA (0,63±0,76 mIU/L) (p=0,639). Disamping itu, tidak juga terdapat perbedaan bermakna rerata kadar FT4 baik pada pemeriksaan menggunakan metode ECLIA (1,67±1,05 ng/dl) maupun metode FIA (1,69±1,72 ng/dl) (p=0,745).

Kesimpulan: Tidak ada perbedaan yang bermakna antara kadar TSH dan FT4 pada metode ECLIA dan FIA.

References

Stathatos N. Thyroid physiology. Med Clin North Am. 2012;96(2):165-173.

INFODATIN. Situasi dan Analisis Penyakit Tiroid. Pusat Data dan Informasi Kementerian Kesehatan RI. 2015.

Kravets I. Hyperthyroidism: Diagnosis and Treatment. Am Fam Physician. 2016;93(5):363-370.

Liewendahl K. Assessment of thyroid status by laboratory methods: developments and perspectives. Scand J Clin Lab Invest Suppl. 1990;201:83-92.

Kazerouni F, Amirrasouli H. Performance characteristics of three automated immunoassays for thyroid hormones. Caspian J Intern Med. 2012;3(2):400-104.

Park HS, Yoo J, Lee H, Choi AR, Ryu J, Park KH, et al. Analytical Evaluation of Portable and Simple FREND Fluorescent Immunoassay for Rapid Quantification of Thyroid-Stimulating Hormone and Free Thyroxine. Clin Lab. 2016;62(12):2455-2460.

Nano Entek. Insert kit FRENDTM thyroid DUO Korea. 2018. [Available at www.nanoentek.com]

Roche. Elecsys Brahms TSH and FT4 Diagnostics Roche. 2018. [Available at http://e-labdoc.roche.com.Germany]

Kahaly GJ, Bartalena L, Hegedüs L, Leenhardt L, Poppe K, Pearce SH. 2018 European Thyroid Association Guideline for the Management of Graves' Hyperthyroidism. Eur Thyroid J. 2018;7(4):167-186.

Carmona CAC, Bedoya PA, Acevedo JB, Arias JAC. Prevalence of Thyroid Disorders in an Institution Providing Health Services in Medellin-Colombia. Translational Biomedicine. 2018;9(2):1-6.

Pramila K, Gopinath P, Shanthi KM, Divya M. Analytical Sensitivity of TSH Assays by ELISA and ELFA. National Journal of Basic Medical Sciences. 2016;6(4):157-162.

Wang D, Yu S, Ma C, Li H, Qiu L, Cheng X, et al. Reference intervals for thyroid-stimulating hormone, free thyroxine, and free triiodothyronine in elderly Chinese persons. Clin Chem Lab Med. 2019;57(7):1044-1052.


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