Research Article

Efek pemberian propolis pada fungsi ginjal dan hepar tikus putih (Rattus Norvegicus) yang diinduksi cisplatin

Novriantika Lestari , Fajrianti Haniyah, Annisa Puspa Sari, Liya Agustin Umar, Elvira Yunita

Novriantika Lestari
Departemen Farmakologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Bengkulu, Bengkulu, Indonesia. Email: novriantika.lestari@gmail.com

Fajrianti Haniyah
Program Studi Kedokteran, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Bengkulu, Bengkulu, Indonesia

Annisa Puspa Sari
Program Studi Kedokteran, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Bengkulu, Bengkulu, Indonesia

Liya Agustin Umar
Departemen Biologi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Bengkulu, Bengkulu, Indonesia

Elvira Yunita
Departemen Biokimia, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Bengkulu, Bengkulu, Indonesia
Online First: July 30, 2021 | Cite this Article
Lestari, N., Haniyah, F., Sari, A., Umar, L., Yunita, E. 2021. Efek pemberian propolis pada fungsi ginjal dan hepar tikus putih (Rattus Norvegicus) yang diinduksi cisplatin. Intisari Sains Medis 12(2): 508-512. DOI:10.15562/ism.v12i2.1017


Background: Cisplatin as an anticancer drug results in nephrotoxicity and hepatotoxicity. Accumulation of cisplatin in the body will produce reactive compounds in the form of free radicals which induce oxidative stress  that affects kidney and liver functions. Propolis contains flavonoids, phenolic acid, and Caffeic Acid Phenethyl Ester (CAPE) which have the ability to prevent free radical formation and improve kidney and liver function. This study aims to determine the effect of propolis administration to ameliorate of BUN, creatinine, ALT and AST levels in cisplatin-induced rats (Rattus norvegicus).

Methods: The experimental study was conducted with the pre-post-test with control group design approach. The research sample used 30 white rats which were divided into normal (aquadest), Cisplatin (5mg/kg i.p), and cisplatin+propolis groups. Propolis was given at a dose of 50mg/kg, 100mg/kg and 200mg/kg orally for seven days, four days after cisplatin induction. Rat blood was taken for analysis of Bloof Urea Nitrogen (BUN), Creatinine, ALT, and AST levels. Data were analyzed using SPSS version 26 for Windows.

Results: The results showed that there were significant differences in levels of BUN, Creatinine, ALT and AST between groups (p<0.05). Propolis treatment at doses of 50 mg/kg, 100mg/kg and 200mg/kg can reduce levels of BUN/urea, creatinine, ALT, and AST in rats that have been induced by cisplatin significantly (p<0,05).

Conclusion: Propolis has a therapeutic effect on cisplatin drug-induced nephrotoxicity and hepatotoxicity by affecting the BUN, creatinine, ALT, and AST levels in rats.

 

Latar Belakang: Penggunaan cisplatin sebagai obat antikanker menghasilkan efek nefrotoksisitas dan hepatotoksisitas imbas obat. Akumulasi cisplatin di dalam tubuh akan menghasilkan senyawa reaktif berupa radikal bebas yang menginduksi terjadinya stress oksidatif sehingga akan berpengaruh ke fungsi ginjal dan hepar. Propolis mengandung  flavonoid, asam fenolat, dan Caffeic Acid Phenethyl Ester (CAPE) yang memiliki kemampuan mencegah pembentukan radikal bebas dan memperbaiki fungsi ginjal dan hepar. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian propolis terhadap pemulihan kadar ureum, kreatinin, ALT dan AST darah pada tikus putih (Rattus norvegicus) yang telah diinduksi cisplatin.

Metode: Penelitian eksperimental ini dilakukan dengan pendekatan pre-post-test with control group design. Sampel penelitian menggunakan 30 ekor tikus putih yang dibagi menjadi kelompok normal (aquadest), Cisplatin (5mg/kgbb i.p) dan kelompok cisplatin+propolis. Propolis diberikan dengan dosis 50mg/kgbb, 100mg/kgbb dan 200mg/kgbb secara oral selama tujuh hari, empat hari setelah induksi cisplatin. Darah tikus diambil untuk analisis kadar Blood Urea Nitrogen (BUN), Kreatinin, ALT, dan AST. Data dianalisis dengan SPSS versi 26 untuk Windows.

Hasil: Hasil penelitian menunjukkan terdapat perbedaan kadar BUN, Kreatinin, ALT, dan AST yang bermakna antar kelompok (p<0,05). Pemberian propolis dengan dosis 50 mg/kgBB, 100 mg/kgBB dan 200 mg/kgBB dapat menurunkan kadar BUN/ureum, kreatinin, ALT, dan AST pada tikus yang telah diinduksi cisplatin secara bermakna (p<0,05).

Kesimpulan: Propolis memiliki efek terapi pada nefrotoksisitas dan hepatotoksisitas akibat obat cisplatin dengan mempengaruhi kadar BUN/ureum, kreatinin, ALT, dan AST pada tikus.

References

Singh TD, Meitei HT, Sharma AL, Robinson A, Singh LS, Singh TR. Anticancer properties and enhancement of therapeutic potential of cisplatin by leaf extract of Zanthoxylum armatum DC. Biol Res. 2015;48(1):46.

Dasari S, Tchounwou PB. Cisplatin in cancer therapy: molecular mechanisms of action. Eur J Pharmacol. 2014;740:364-378.

Perše M, Ve?eri?-Haler Ž. Cisplatin-Induced Rodent Model of Kidney Injury: Characteristics and Challenges. Biomed Res Int. 2018;2018:1462802.

Mir M, Arab MR, Shahraki MR, Mashhadi MA, Salar MS, Aval FS, et al Toxic Effects of Cisplatin on Hepatocytes and Liver Enzymes of Rats. Anatomical Sciences. 2015;12(4):171-176.

Naqshbandi A, Khan MW, Rizwan S, Rehman SU, Khan F. Studies on the protective effect of dietary fish oil on cisplatin induced nephrotoxicity in rats. Food Chem Toxicol. 2012;50(2):265-273.

Miller RP, Tadagavadi RK, Ramesh G, Reeves WB. Mechanisms of Cisplatin nephrotoxicity. Toxins (Basel). 2010;2(11):2490-2518.

Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M, Swierczek-Zieba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules. 2013;19(1):78-101.

Bankova VS, de Castro SL, Marcucci MC. Propolis: Recent advances in chemistry and plant origin. Apidologie. 2000;31(1):3–15

Higuchi K, Yanagawa T. Evaluating dose of cisplatin responsible for causing nephrotoxicity. PLoS One. 2019;14(4):e0215757.

Bhadauria M. Combined treatment of HEDTA and propolis prevents aluminum induced toxicity in rats. Food Chem Toxicol. 2012;50(7):2487-2495.

Bentli R, Parlakpinar H, Polat A, Samdanci E, Sarihan ME, Sagir M. Molsidomine prevents cisplatin-induced hepatotoxicity. Arch Med Res. 2013;44(7):521-528.

El Menyiy N, Al-Waili N, El Ghouizi A, Al-Waili W, Lyoussi B. Evaluation of antiproteinuric and hepato-renal protective activities of propolis in paracetamol toxicity in rats. Nutr Res Pract. 2018;12(6):535-540.

Ibrahim NA. The possible protective effect of bee propolis on experimentally mediated cisplatin reproductive toxicity: a histological and immunohistochemical study. The Egyptian Journal of Histology. 2013;36(1):78-86.

Barkin RM. Toxicologic emergencies. Pediatr Ann. 1990;19(11):629-633.

Perše M, Ve?eri?-Haler Ž. Cisplatin-Induced Rodent Model of Kidney Injury: Characteristics and Challenges. Biomed Res Int. 2018;2018:1462802.

Sales GTM, Foresto RD. Drug-induced nephrotoxicity. Rev Assoc Med Bras (1992). 2020;66Suppl 1(Suppl 1):s82-s90.

Weiner ID, Mitch WE, Sands JM. Urea and Ammonia Metabolism and the Control of Renal Nitrogen Excretion. Clin J Am Soc Nephrol. 2015;10(8):1444-1458.

Hediger MA, Smith CP, You G, Lee WS, Kanai Y, Shayakul C. Structure, regulation and physiological roles of urea transporters. Kidney Int. 1996;49(6):1615-1623.

Cüre MC, Cüre E, Kalkan Y, et al. Infliximab Modulates Cisplatin-Induced Hepatotoxicity in Rats. Balkan Med J. 2016;33(5):504-511.

Goorden SM, Buffart TE, Bakker A, Buijs MM. Liver disorders in adults: ALT and AST. Ned Tijdschr Geneeskd. 2013;157(43):A6443.

Gowda S, Desai PB, Hull VV, Math AA, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. Pan Afr Med J. 2009;3:17.

Kumar S, Pandey AK. Chemistry and biological activities of flavonoids: an overview. ScientificWorldJournal. 2013;2013:162750.

Altunta? A, Y?lmaz HR, Altunta? A, Uz E, Demir M, Gökçimen A, et al. Caffeic acid phenethyl ester protects against amphotericin B induced nephrotoxicity in rat model. Biomed Res Int. 2014;2014:702981.

Khairunnisa K, Mardawati E, Putri SH. Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Propolis Lebah Trigona Sp. Jurnal Industri Pertanian. 2020;2(1):124-129.

Vargas F, Romecín P, García-Guillén AI, Wangesteen R, Vargas-Tendero P, Paredes MD, et al. Flavonoids in Kidney Health and Disease. Front Physiol. 2018;9:394.

Ogeturk M, Kus I, Colakoglu N, Zararsiz I, Ilhan N, Sarsilmaz M. Caffeic acid phenethyl ester protects kidneys against carbon tetrachloride toxicity in rats. J Ethnopharmacol. 2005;97(2):273-280.

Kaya E, Y?lmaz S, Ceribasi S. Protective Role of Propolis on Low and High Dose Furan-induced Hepatotoxicity and Oxidative Stress in Rats. J Vet Res. 2019;63(3):423-431.

Talas ZS, Gogebakan A, Orun I. Effects of propolis on blood biochemical and hematological parameters in nitric oxide synthase inhibited rats by N?-Nitro-L-arginine methyl ester. Pak J Pharm Sci. 2013;26(5):915-919.


Article Views      : 45
PDF Downloads : 19