Research Article

Pengaruh lama paparan trauma listrik terhadap sistem saraf traktus gastrointestinal pada tikus Wistar galur murni

Slamet Rahardja , Adeodatus Yuda Handaya, Agus Barmawi

Slamet Rahardja
Trainee Bedah Digestif, Fakultas Kedokteran Universitas Gadjah Mada, RSUP Dr Sardjito Yogyakarta, Indonesia. Email: adeng.slametrahardja@gmail.com

Adeodatus Yuda Handaya
Konsultan Bedah Digestif, Fakultas Kedokteran Universitas Gadjah Mada, RSUP Dr Sardjito Yogyakarta, Indonesia

Agus Barmawi
Konsultan Bedah Digestif, Fakultas Kedokteran Universitas Gadjah Mada, RSUP Dr Sardjito Yogyakarta, Indonesia
Online First: July 30, 2021 | Cite this Article
Rahardja, S., Handaya, A., Barmawi, A. 2021. Pengaruh lama paparan trauma listrik terhadap sistem saraf traktus gastrointestinal pada tikus Wistar galur murni. Intisari Sains Medis 12(2): 504-507. DOI:10.15562/ism.v12i2.1001


Background: Electrical trauma can cause injury to the body tissues it passes through, causing death. The injury is part of the process of cell damage, including the digestive organs. This study aims to determine the effect of the duration of electrical trauma on the nervous system of the gastrointestinal tract histopathologically.

Methods: This study was experimental with the research subjects were 24 pure strain Wistar rats, which were divided into 4 groups. The first group is the control group (K) without treatment. Then, group (X) was exposed to electricity for 15 seconds, group (Y) was given exposure for 30 seconds, and group (Z) was exposed to 60 seconds. From the preparations, the number of nerve cells was counted. Data were analyzed with SPSS version 20 for Windows.

Results: There was a significant effect on the duration of electrical trauma on the number of the gastrointestinal tract nervous system histopathologically (p<0.05). In the control group (K), the number of nerve cells for the stomach was 91.33±0.60, followed by the ileum (89.50±07.09), and colon (94.33±6.18). In group (X), the number of nerve cells in the stomach was 79.50±11.823, followed by the ileum (62.00±15.31), and colon (78.83±5.04). In group (Y), the number of nerve cells for gastric was 45.33±7.66, followed by ileum (34.00±16.27), and colon (47.17±9.56). Meanwhile, in the group (Z), the number of nerve cells for the stomach was 2.40±0.55, followed by the ileum (1.40±1.52), and colon (1.80±1.10). Thus, there was a significant difference between the study groups (p<0.001).

Conclusion: The duration of electrical trauma significantly affects the number of the gastrointestinal tract nervous system histopathologically.

 

 

Latar Belakang: Trauma listrik dapat menimbulkan perlukaan pada jaringan tubuh yang dilewatinya hingga menyebabkan kematian. Perlukaan tersebut merupakan bagian dari proses kerusakan sel, termasuk pada organ digestif. Penelitian ini bertujuan untuk mengetahui pengaruh lama paparan trauma listrik terhadap sistem saraf traktus gastrointestinal secara histopatologi.

Metode: Penelitian ini bersifat eksperimental dengan subyek penelitian adalah 24 ekor tikus Wistar galur murni, yang terbagi dalam 4 kelompok. Kelompok pertama merupakan kelompok kontrol (K) tanpa perlakuan. Kelompok (X) diberi paparan listrik selama 15 detik, kelompok (Y) diberi paparan selama 30 detik, dan kelompok (Z) diberi paparan selama 60 detik. Dari preparat, dilakukan penghitungan terhadap jumlah sel saraf. Data dianalisis dengan SPSS versi 20 untuk Windows.

Hasil: Terdapat pengaruh yang bermakna pada lama trauma listrik terhadap jumlah sistem saraf traktus gastrointestinal secara histopatologis (p<0,05). Pada kelompok kontrol (K), jumlah sel saraf untuk gaster adalah 91,33±0,60, diikuti dengan ileum (89,50±07,09), dan kolon (94,33±6,18). Pada kelompok (X), jumlah sel saraf pada gaster adalah 79,50±11,823, diikuti dengan ileum (62,00±15,31), dan kolon (78.83±5.04). Pada kelompok (Y), jumlah sel saraf untuk gaster adalah 45,33±7,66, diikuti dengan ileum (34,00±16,27), dan kolon (47,17±9,56). Sedangkan pada kelompok (Z), jumlah sel saraf untuk gaster adalah 2,40±0,55, diikuti dengan ileum (1,40±1,52), dan kolon (1,80±1,10). Didapatkan perbedaan bermakna  antara kelompok penelitian (p<0,001).

Kesimpulan: Lama trauma listrik memiliki pengaruh secara bermakna terhadap jumlah sistem saraf traktus gastrointestinal secara histopatologi.

References

Koumbourlis AC. Electrical injuries. Crit Care Med. 2002;30(11 Suppl):S424-S430.

Kym D, Seo DK, Hur GY, Lee JW. Epidemiology of electrical injury: Differences between low- and high-voltage electrical injuries during a 7-year study period in South Korea. Scand J Surg. 2015;104(2):108-114.

Shih JG, Shahrokhi S, Jeschke MG. Review of Adult Electrical Burn Injury Outcomes Worldwide: An Analysis of Low-Voltage vs High-Voltage Electrical Injury. J Burn Care Res. 2017;38(1):e293-e298.

Martinez JA, Nguyen T. Electrical injuries. South Med J. 2000;93(12):1165-1168.

Buniak B, Reedy DW, Caldarella FA, Bales CR, Buniak L, Janicek D. Alteration in gastrointestinal and neurological function after electrical injury: a review of four cases. Am J Gastroenterol. 1999;94(6):1532-1536.

Esses SI, Peters WJ. Electrical burns; pathophysiology and complications. Can J Surg. 1981;24(1):11-14.

Marques EG, Júnior GA, Neto BF, Freitas RA, Yaegashi LB, Almeida CE, et al. Visceral injury in electrical shock trauma: proposed guideline for the management of abdominal electrocution and literature review. Int J Burns Trauma. 2014;4(1):1-6.

Gulbransen BD, Sharkey KA. Novel functional roles for enteric glia in the gastrointestinal tract. Nat Rev Gastroenterol Hepatol. 2012;9(11):625-632.

Oommen V, Kanthakumar P. The gastrointestinal system: a piece of cake. Adv Physiol Educ. 2015;39(2):128.

Szurszewski JH. A 100-year perspective on gastrointestinal motility. Am J Physiol. 1998;274(3):G447-G453.

Gershon MD. Review article: serotonin receptors and transporters -- roles in normal and abnormal gastrointestinal motility. Aliment Pharmacol Ther. 2004;20 Suppl 7:3-14.

Li Z, Hao MM, Van den Haute C, Baekelandt V, Boesmans W, Vanden Berghe P. Regional complexity in enteric neuron wiring reflects diversity of motility patterns in the mouse large intestine. Elife. 2019;8:e42914.

Gershon MD. The enteric nervous system: a second brain. Hosp Pract (1995). 1999;34(7):31-42.

Hao MM, Young HM. Development of enteric neuron diversity. J Cell Mol Med. 2009;13(7):1193-1210.

Uzün I, Akyildiz E, Inanici MA. Histopathological differentiation of skin lesions caused by electrocution, flame burns and abrasion. Forensic Sci Int. 2008;178(2-3):157-161.

Bassotti G, Villanacci V, Fisogni S, Rossi E, Baronio P, Clerici C, et al. Enteric glial cells and their role in gastrointestinal motor abnormalities: introducing the neuro-gliopathies. World J Gastroenterol. 2007;13(30):4035-41.

Leibovici D, Shemer J, Shapira SC. Electrical injuries: current concepts. Injury. 1995;26(9):623-627.

Williams DB, Karl RC. Intestinal injury associated with low-voltage electrocution. J Trauma. 1981;21(3):246-250.

Narwanto I, Aswin S, Mustafa. Pengaruh Pemberian Etanol secara Kronik Terhadap Jumlah Sel Piramidal di Ca1 Hippocampus Tikus (Rattus Norvegicus) Remaja. Jurnal Anatomi Indonesia. 2007;2(1):29-33.

Rohmah IN, Sadad AR, Johan A. Perbedaan Jumlah Sel Nekrosis Sel Otak dan Nilai pH Darah Tikus Wistar pada Pemberian Formalin Peroral Dosis Bertingkat. Media Medika Muda. 2016;1(1):31-37.

Rizal S. Perbedaan Gambaran Histopatologi Otak Tikus Wistar Akibat Paparan Arus Listrik pada Media Air Tawar dan Air Laut [Skripsi]. Program Pendidikan Sarjana Kedokteran Universitas Diponegoro. 2014

Branday JM, DuQuesnay DR, Yeesing MT, Duncan ND. Visceral complications of electrical burn injury. A report of two cases and review of the literature. West Indian Med J. 1989;38(2):110-113.

Sharma M, Kaundal P, Sharma P, Chaudhary R. Electric Current Causing Sigmoid Perforation: Case Report. Annals of International Medical and Dental Research. 2015;1(1):39-40.


No Supplementary Material available for this article.
Article Views      : 184
PDF Downloads : 68